# Cell and Molecular Imaging Core

> **NIH NIH P30** · MEDICAL UNIVERSITY OF SOUTH CAROLINA · 2021 · $195,704

## Abstract

Cell & Molecular Redox Imaging Core – Project Summary
The South Carolina COBRE in Oxidants, Redox Balance and Stress Signaling (Redox COBRE) Cell and
Molecular Imaging Redox Core (CMRI) provides advanced technologies and expertise (Aim 1), capabilities for
new method development (Aim 2), and training (Aim 3) to target investigators and Center members for state-of-
the-art cell- and tissue-based microscopic imaging. This includes confocal, multiphoton and super-resolution
microscopy of live and fixed cells and tissues, intravital imaging, and automated imaging of histological slides.
Additionally, the Core maintains and develops a variety of molecular tools and redox indicators for use in COBRE
investigations. The CMRI houses the following major microscope systems: 1) Zeiss LSM 880 NLO
multiphoton/confocal system equipped with a Coherent Chameleon multi-photon laser, Quasar spectral
detection and Airyscan super-resolution capability; 2) Olympus Fluoview FV1200 multiphoton microscope with
SpectraPhysics MaiTai DeepSee laser and silicone oil optics for intravital imaging; 3) Olympus Fluoview FV 10i
LIV live cell confocal microscope with water immersion optics; 4) Zeiss LSM 510 META confocal microscope; 5)
BD BioSciences CARV II disk-scanning confocal microscope for video-rate imaging; 6) Zeiss Axiovert 200M
wide-field fluorescence microscope; and 7) Perkin-Elmer Vectra Polaris Automated Quantitative Pathology
Imaging System. Except for the Vectra Polaris, which is customized for histological slides, all microscopes are
equipped with environmental chambers for temperature and gas phase control to allow non-destructive 3D
imaging of living cells, tissues and organisms. Major applications include: 1) live cell imaging of parameter-
sensitive fluorophores to monitor ions, electrical potentials, radical generation, pyridine nucleotide reduction,
membrane permeability, cell viability (apoptosis and necrosis), and the submicron distribution of fluorescent
proteins and other fluorescent reporters; 2) high resolution imaging of tissue sections for immunocytochemistry
and fluorescent protein distribution; 3) fluorescence resonance energy transfer (FRET) and DuoLink to
characterize and quantify interactions between specific molecules; 4) intravital microscopy to monitor
microcirculation, leukocyte margination, mitochondrial polarization and permeability, radical generation, gene
expression and other parameters in living animals; and 5) high throughput, quantitative multiplexed imaging of
conventionally and immunostained clinical and research specimens. Ancillary equipment required for specimen
preparation is also provided. Consultation and services for transmission and scanning electron microscopy (TEM
and SEM, respectively) are available through the Department of Pathology & Laboratory Medicine. Computer
workstations provide offline image processing/analysis (ImageJ FIJI, Metamorph, IPLab and other software).

## Key facts

- **NIH application ID:** 10174250
- **Project number:** 1P30GM140964-01
- **Recipient organization:** MEDICAL UNIVERSITY OF SOUTH CAROLINA
- **Principal Investigator:** John J Lemasters
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $195,704
- **Award type:** 1
- **Project period:** 2021-08-01 → 2026-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10174250

## Citation

> US National Institutes of Health, RePORTER application 10174250, Cell and Molecular Imaging Core (1P30GM140964-01). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10174250. Licensed CC0.

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