Analytical Redox Core – Project Summary The South Carolina COBRE in Oxidants, Redox Balance and Stress Signaling (Redox COBRE) Analytical Redox Core (ARC) offers infrastructure, expertise and training to Center members to support the successful application of quantitative techniques for the study of redox homeostasis and cellular bioenergetics. This includes unique approaches, technologies and equipment specific to the detection of redox-sensitive molecules and enzymes that drive redox homeostasis. The aims of the ARC are to: provide investigators with advanced capabilities in quantitative analytical redox biochemistry; state-of-the-art bioenergetic profiling; analysis and characterization of redox signaling pathways; and in particular, expert mentoring, education, training and consultation with COBRE personnel are top priorities of the ARC. Thus, we are able to impart the importance of redox signaling pathways in human disease, ensuring ongoing Core utilization and development of our user base, while enabling scientific discovery by COBRE investigators and the greater MUSC research community. During Phase III, we will integrate key resources (e.g., Seahorse XF bioenergetics profiling assays) previously available through the Bioenergetics Core that is now no longer available. Combined with our expertise in analytical biochemistry, the Phase III ARC aims to provide COBRE members with a multidisciplinary and comprehensive suite of services for quantifying ROS/RNS species and redox-modified proteins as well as characterization of their impact on bioenergetic networks and pathobiological processes.