# Microbiology Capability/Capacity Development

> **NIH FDA U19** · NEW JERSEY STATE DEPARTMENTR/AGRICULTURE · 2020 · $105,000

## Abstract

Project Summary 
(Microbiology: Capability/Capacity Development, Whole Genome Sequencing) 
The New Jersey Department of Agriculture (NJDA) laboratory’s aim, in participating in the 
microbiology capability/capacity development project, is to develop next generation sequencing 
(NGS) capability of food borne pathogens and related veterinary clinical isolates. The NJDA 
microbiology program has resources including facility, equipment, and personnel to isolate 
pathogenic microorganisms from samples. The NJDA microbiology program lacks equipment 
required to accomplish the whole genome sequencing. To this end, the NJDA would like to 
purchase an Illumina iSEQ 100 next generation sequencer, reagents, consumables, and 
software, and obtain training in whole genome sequencing (WGS). Microbial sequencing on the 
iSEQ 100 is part of an integrated workflow system that consists of library preparation with the 
Nextera DNA Flex Library preparation kit, Illumina sequencing, and push button data analysis in 
Base Space Sequence hub. Laboratory personnel will attend online and onsite WGS and 
bioinformatics trainings and pass an initial competency assessment before proceeding to do the 
WGS. Personnel will also participate in GenomeTrakr proficiency tests and attend the annual 
GenomeTrakr and face-to-face meeting. The acquisition of next generation sequencer along with 
all supplies, consumables, and personnel will greatly enhance the NJDA laboratory’s capability 
and the national capacity.

## Key facts

- **NIH application ID:** 10174549
- **Project number:** 1U19FD007075-01
- **Recipient organization:** NEW JERSEY STATE DEPARTMENTR/AGRICULTURE
- **Principal Investigator:** Amar Patil
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** FDA
- **Fiscal year:** 2020
- **Award amount:** $105,000
- **Award type:** 1
- **Project period:** 2020-09-01 → 2025-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10174549

## Citation

> US National Institutes of Health, RePORTER application 10174549, Microbiology Capability/Capacity Development (1U19FD007075-01). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10174549. Licensed CC0.

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