# Core B: High-Throughput Biology

> **NIH NIH P01** · UNIVERSITY OF PENNSYLVANIA · 2021 · $204,523

## Abstract

The High-throughput Biology Core will express recombinant proteins and protein complexes, purify 
recombinant proteins to homogeneity, and produce high-titer stocks of lentiviruses for delivery of cDNAs, 
shRNA and CRISPR/dCAS9 to (epi)genetically manipulate cells in vitro. Furthermore, the Core will 
support high-throughput screening to identify genes and small molecule inhibitors, respectively, of key 
signaling pathways that regulate protein functions or phenotypes associated with cellular senescence 
and/or age-related pathologies in tissues, including the pro-aging senescence-associated secretory 
phenotype (SASP). The centralization of these services under the direction of an experienced core 
leader who has been a long standing member of this PO1, highly trained technical staff, and the unique 
laboratory infrastructure, including robotic pipetting workstations, a multi-label plate readers, a high- 
content screening reader, small molecule and genetic libraries, minimizes redundancy; maximizes 
biosafety associated with recombinant DNA technologies; ensures preparation of high-quality biological 
materials for seamless integration of reagents across projects; preserves the time of project staff to 
focus on experimentation to test key hypotheses; and enables investigators to use unbiased approaches 
to identify new genes and small molecules that regulate protein function or cellular processes. 
Collectively, these attributes of the proposed core will enable high-throughput, economic, and rapidly 
scalable services to produce high-quality biological materials. Based on progress from the current 
funding period and usage of the proposed research activities, the core will continue to be an integral 
component of the overall program by generating many recombinant protein reagents, viral vectors, and 
small molecule inhibitors that will facilitate long-term programmatic interactions and enable central 
hypotheses of this program project to be tested.

## Key facts

- **NIH application ID:** 10174635
- **Project number:** 5P01AG031862-14
- **Recipient organization:** UNIVERSITY OF PENNSYLVANIA
- **Principal Investigator:** DAVID C SCHULTZ
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $204,523
- **Award type:** 5
- **Project period:** 2008-03-15 → 2023-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10174635

## Citation

> US National Institutes of Health, RePORTER application 10174635, Core B: High-Throughput Biology (5P01AG031862-14). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10174635. Licensed CC0.

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