# The Origin of Rod Shape in Bacteria

> **NIH NIH R01** · TEXAS A&M UNIVERSITY · 2021 · $307,492

## Abstract

A fundamental question in biology that remains to be understood is how cell shapes are genetically
encoded and enzymatically generated. Spheres and rods are among the most common shapes adopted by
walled bacteria. The bacterial cell wall, formed by peptidoglycan, largely determines cell shape. When bacteria
reproduce by binary fission (division), the shapes of the two daughter cells are already determined by their
mother cell. For this reason, it is difficult to explore the origin of cell shape using healthy bacterial cells. Taking
advantage of a sphere-to-rod morphological transition during the germination of the bacterium Myxococcus
xanthus, we propose to explore the mechanisms underlying the generation and maintenance of rod-like cell
shapes. Cells of M. xanthus change back and forth between rods and spheres at different life stages. Rod-
shaped vegetative cells thoroughly degrade their walls and shrink into uniformly sized spherical cells that then
mature into spores. Unlike endospore formation in Bacillus species, whole M. xanthus cells convert to spores.
As the spores of M. xanthus germinate, cells rebuild their walls and reestablish rod shape without preexisting
templates. This unique morphological transition provides a rare opportunity to visualize de novo cell wall
synthesis (CWS) and bacterial morphogenesis. To understand the mechanism of morphogenesis in M.
xanthus, we propose three specific aims. First, to reveal the mechanism of de novo CWS during germination,
we will test the hypothesis that a defined pattern of cell wall growth is required for the establishment of rod
shape. Second, to elucidate the mechanism by which the MreB cytoskeleton supports rod shape during
germination, we will test the hypothesis that MreB determines rod shape by guiding the motion of the CWS
machinery. Third, to reveal the mechanism by which cell polarity regulates CWS and rod shape in germinating
spores, we will test the hypothesis that the establishment of cell polarity is necessary for the sphere-to-rod
transition. Because the peptidoglycan cell wall is widely conserved among bacteria, we expect that our results
with M. xanthus will reveal fundamental mechanisms of morphogenesis, which is difficult to study in other
bacteria.

## Key facts

- **NIH application ID:** 10174955
- **Project number:** 5R01GM129000-04
- **Recipient organization:** TEXAS A&M UNIVERSITY
- **Principal Investigator:** Beiyan Nan
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $307,492
- **Award type:** 5
- **Project period:** 2018-09-01 → 2023-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10174955

## Citation

> US National Institutes of Health, RePORTER application 10174955, The Origin of Rod Shape in Bacteria (5R01GM129000-04). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10174955. Licensed CC0.

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