# Injectable Carbon Nanotube-Functionalized Hydrogel for miRNA Delivery

> **NIH NIH K25** · UNIVERSITY OF COLORADO DENVER · 2021 · $178,243

## Abstract

ABSTRACT
microRNAs (miRNAs) have been proven to promote cardiac regeneration after myocardial infarction. However,
current miRNA delivery methods, such as viral vectors or lipid formulations, present safety concerns for
widespread use. We have developed an injectable thermo-responsive hydrogel functionalized with carbon
nanotubes (RTG-CNT) for the delivery of miRNAs. The RTG-CNT hydrogel transitions from a liquid-solution to
a gel-based matrix shortly after reaching body temperature allowing for a liquid-based delivery rapidly followed
by a stable-gel miRNA localization. Moreover, this hydrogel has improved short-term (8-week) biocompatibility
compared to viral and lipid approaches and it promotes two-fold more miRNA expression than lipid formulations.
In this investigation, we propose to test the hypothesis that our novel RTG-CNT hydrogel is far superior
delivery model of miRNAs to the heart, through increased biocompatibility, targeted delivery and higher
miRNA expression when compared to viral and lipid approaches. We will address our hypothesis with a
combination of cell biology and bioengineering by 1) Quantify the biocompatibility and the magnitude of improved
localization of our RTG-CNT-miRNA delivery system over liposomal and viral vectors approaches, 2) Measure
the improved efficiency of the RTG-CNT hydrogel as pro-regenerative miRNA delivery system vs. liposomal and
viral vector deliveries in a mouse MI model and 3) Determine the potential of the RTG-CNT hydrogel to deliver
anti-fibrotic miRNAs to further improve myocardial structure and rescue function in a mouse MI model. We
believe that the RTG-CNT hydrogel will offer a more biocompatible and far more efficient miRNA delivery system
than traditional approaches, that can be realistically translated into clinical applications.

## Key facts

- **NIH application ID:** 10175017
- **Project number:** 5K25HL148386-02
- **Recipient organization:** UNIVERSITY OF COLORADO DENVER
- **Principal Investigator:** Brisa Marisol Pena Castellanos
- **Activity code:** K25 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $178,243
- **Award type:** 5
- **Project period:** 2020-06-01 → 2025-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10175017

## Citation

> US National Institutes of Health, RePORTER application 10175017, Injectable Carbon Nanotube-Functionalized Hydrogel for miRNA Delivery (5K25HL148386-02). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10175017. Licensed CC0.

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