# IDALS LFFM Special Projects Method Development Validation

> **NIH FDA U19** · IOWA DEPT AGRICULTURE/LAND STEWARDSHIP · 2020 · $34,996

## Abstract

Project Summary Special Projects – Method Development/Validation 
This project is a matrix extension single laboratory validation that will expand testing for chemical 
hazards (pesticides) into animal feed. IDALS has been using a modified multi-residue method, based on 
QuEChERS extraction coupled with high-performance liquid chromatography–tandem mass 
spectrometry (LC-MS/MS) for detecting residue levels of acid herbicides. The QuEChERS method has 
extraction and clean-up steps, which have been developed to be Quick, Easy, Cheap, Efficient, Rugged 
and Safe. Originally, the modified method was validated for plant matrices but since then has been 
successfully adapted for soil and swabs with reasonably high recovery rates. With the surge of 
importance of detecting herbicide residues in feed, IDALS proposes to extend the scope of this multi- 
residue pesticide method by conducting a matrix extension by executing a level two single laboratory 
validation for acid herbicides with animal feed. A method to detect the presence of pesticides in animal 
feeds will support FDA efforts to minimize foodborne exposure to hazardous chemicals for both animals 
and humans.

## Key facts

- **NIH application ID:** 10175211
- **Project number:** 1U19FD007120-01
- **Recipient organization:** IOWA DEPT AGRICULTURE/LAND STEWARDSHIP
- **Principal Investigator:** TRAVIS JAY KNIGHT
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** FDA
- **Fiscal year:** 2020
- **Award amount:** $34,996
- **Award type:** 1
- **Project period:** 2020-09-01 → 2025-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10175211

## Citation

> US National Institutes of Health, RePORTER application 10175211, IDALS LFFM Special Projects Method Development Validation (1U19FD007120-01). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10175211. Licensed CC0.

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