# Fusion Lumos Orbitrap System for Quantitative Proteomics and Metabolomics Applications

> **NIH NIH S10** · UNIVERSITY OF COLORADO DENVER · 2021 · $1,272,480

## Abstract

Project Summary
This application is for an Orbitrap Lumos Fusion liquid chromatography mass spectrometer system to be housed
within the Mass Spectrometry Facility at the Skaggs School of Pharmacy and Pharmaceutical Sciences (SSPPS)
at University of Colorado, Anschutz Medical Campus. This instrument will be used to advance several
translational projects that focus on quantitative and discovery-based proteomics and the analysis of post-
translational modifications (PTMs), such as acetylation, with direct application to human health and disease. The
instrument will also be used for metabolomics projects that require high resolving power to improve identification
of unknown compounds. The Users’ projects are currently being conducted on older, Q-TOF instrumentation
that cannot acquire the depth of proteome coverage or resolution required for multiplex proteomics experiments,
PTM analysis, or fine molecular structure elucidation. Hence there is an urgent need for this instrumentation.
The Orbitrap Lumos has several critical features for the intended applications including exceptional sensitivity,
ultra high resolving power, MSn capabilities, electron transfer dissociation (ETD), versatility in quantitative
capabilities, and well-published methods. Dr. David Ross will utilize the Orbitrap Lumos to reveal mechanisms
underlying insulin sensitivity through in-depth, quantitative proteomics and PTM analysis using ETD, with the
potential to impact therapies directed at metabolic syndrome. Dr. Thomas Delong will employ ETD for de novo
hybrid peptide discovery to examine immunological origins of certain diseases, such as diabetes and Alzheimer’s
disease. Dr. Anchordoquy will require the instrument to achieve deep proteome, metabolome, and lipidome
coverage of extracellular vesicles towards improved drug delivery systems. Dr. Brown requires a highly sensitive
and specific redox proteomics approach to understanding regulation of mast cell activation. Finally, the
instrument will be used by Dr. Reisdorph and others to support the identification of small molecules using its
capabilities in multi-stage experiments, MSn, high resolution, and accurate mass. Our long-term objective is to
utilize the Orbitrap Lumos to further develop our programs in acetylomics, PTM quantitation, and metabolomics.
As our Core currently lacks any type of Orbitrap technology, our proteomics and metabolomics services have hit
a plateau; cutting edge instrumentation is required to take the research of our faculty to the next level. The
SSPPS has committed over $500,000 (not including equipment) in the past 5 years towards the improvements
of mass spectrometry facility, which has been supported by SSPPS for almost 20 years. Our objective is to
continue to provide our investigators with a shared mass spectrometry resource to support current and proposed
NIH-funded biomedical research. This Orbitrap is vital to the continued functioning of the facility as a cutting-
edge enterprise and for t...

## Key facts

- **NIH application ID:** 10175908
- **Project number:** 1S10OD028538-01A1
- **Recipient organization:** UNIVERSITY OF COLORADO DENVER
- **Principal Investigator:** Nichole A Reisdorph
- **Activity code:** S10 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $1,272,480
- **Award type:** 1
- **Project period:** 2021-07-15 → 2022-07-14

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10175908

## Citation

> US National Institutes of Health, RePORTER application 10175908, Fusion Lumos Orbitrap System for Quantitative Proteomics and Metabolomics Applications (1S10OD028538-01A1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10175908. Licensed CC0.

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