# ZellScanner ONE

> **NIH NIH S10** · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · 2021 · $286,800

## Abstract

Project Summary/Abstract
This request is for funds to purchase a Canopy Biosciences ZellScanner One high-multiplexing chip 
cytometer. There is no ZellScanner imaging platform at the University of Pittsburgh or within 100 
miles. Unique as an instrument, the ZellScanner system combines the strengths of 
multiparameter flow cytometry (marker co-expression, quantitative, sensitive) with 
microscopy (positional information, tissue architecture, subcellular detail) to permit analysis 
of highly defined immune subsets and their spatial positioning in the native tissue 
microenvironment. Such high content imaging allows for deep insights into immune cell 
subpopulations in the context of tissue localization. The instrument will be housed within the 
Unified Flow Cytometry Core, a centralized core, at the University of Pittsburgh School 
of Medicine. The Unified Flow Core currently serves over 180 Pis and 500 individual Users. For the 
proposed instrument, Major Users include 13 Pis (10 NiH funded) with another 5 Pis as Minor Users. 
Together, these Pis have over $20 M in NiH-funded direct costs from at least 4 
different institutes. A major research focus is in the broad area of immunology and allied immune 
diseases, with sister disciplines such as transplantation, rheumatology, vascular biology, 
and oncology also well represented. While the Unified Flow Cytometry Core is well-endowed with 
traditional (though busy) analyzers and sorters, the ZellScanner chip cytometer brings 
totally new breadth to our research capabilities. Our preliminary and demo work proved 
to us that this instrument will truly allow our investigators to break new ground in their 
research. The demo phase of this instrument indeed created great excitement among the Pis and 
hands-on Users. The new machine will be well cared for by the staff of the Unified Flow Cytometry 
Core, which has an extensive infrastructure and culture in place to train Users, oversee the 
machine and ensure that it is self-supporting. Two experienced core personnel will be responsible 
for the conceptual and day to day aspects of experiments on the ZellScanner unit. We will start and 
certify the machine each morning and troubleshoot any problems, as we will do at any time during 
working hours. As such, it will have a remarkable impact on the productivity of an 
outstanding and well-funded cohort of PHS investigators.

## Key facts

- **NIH application ID:** 10176806
- **Project number:** 1S10OD030396-01
- **Recipient organization:** UNIVERSITY OF PITTSBURGH AT PITTSBURGH
- **Principal Investigator:** Lisa Borghesi
- **Activity code:** S10 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $286,800
- **Award type:** 1
- **Project period:** 2021-05-01 → 2022-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10176806

## Citation

> US National Institutes of Health, RePORTER application 10176806, ZellScanner ONE (1S10OD030396-01). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10176806. Licensed CC0.

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