# Microscale Thermophoresis

> **NIH NIH S10** · NORTHEASTERN UNIVERSITY · 2021 · $368,431

## Abstract

Project Summary
We propose to acquire a NanoTemper Monolith NT-Auto, with detection of both fluorescently-labeled
molecules and of intrinsic fluorescence. This instrument provides maximum flexibility for investigators and will
be the only instrument of its kind in the Boston area. The Monolith instruments measure microscale
thermophoresis, which is based on the generation of a precise temperature gradient while monitoring changes
in sample migration along this gradient, as detected by sample fluorescence to determine binding. The
changes in migration of molecules along the temperature gradient reflect changes in molecular hydration shell,
charge, or size; as one or all of these parameters change upon ligand binding, differences in the bound and
unbound states can be determined. The requested instrument allows determination of binding affinities,
oligomerization, and other parameters without immobilization. The instrument will be used to advance
numerous projects related to cancer, antibiotic resistance, infectious disease, viral replication, inflammation,
and neurological disorders including addiction. A second major focus is on drug design, development, and
delivery.

## Key facts

- **NIH application ID:** 10177014
- **Project number:** 1S10OD030369-01
- **Recipient organization:** NORTHEASTERN UNIVERSITY
- **Principal Investigator:** Jared Auclair
- **Activity code:** S10 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $368,431
- **Award type:** 1
- **Project period:** 2021-04-25 → 2022-04-24

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10177014

## Citation

> US National Institutes of Health, RePORTER application 10177014, Microscale Thermophoresis (1S10OD030369-01). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10177014. Licensed CC0.

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