# Orbitrap Tribrid Mass Spectrometer for Wayne State Proteomics

> **NIH NIH S10** · WAYNE STATE UNIVERSITY · 2021 · $1,289,466

## Abstract

Project Summary/Abstract
The goal of this proposal is to bring a Thermo Fisher Scientific Orbitrap Eclipse mass spectrometer
with Electron Transfer Dissociation (ETD) capability and a High-Field Asymmetric Waveform Ion Mobility
Spectrometry (FAIMS-Pro) devise plus a nano-LC to the Proteomics Core at Wayne State University.
The Proteomics Core supports an extensive user base including 9 Major Users and 16 Minor Users who
have contributed to the proposal and who have NIH-funded projects that will be advanced by having access
to the Orbitrap Eclipse. The Proteomics Core at Wayne State also supports the Karmanos Cancer Institute
and P30 funded Cancer Center as well as the NIEHS funded CURES P30 Center. This Orbitrap Eclipse
has unique capabilities for deep profiling of complex proteomes and isobaric tag based multiplexing
due to the 40 Hz scan speed and the unique feature of Real Time Search that is enabled on the on-
board computer. The computational advances incorporated into the Orbitrap Eclipse make it an
exceptional instrument for proteomic isotopic based quantitation and for newer approaches such as
Data Independent Analysis. Combined with the experienced mass spectrometrists in the Core, the
Orbitrap Eclipse will be a major asset to research programs of NIH-funded investigators at WSU and
in the SE Michigan region. As investigator needs have evolved they have developed an increased need
for higher resolution, greater sensitivity and higher mass accuracy proteomic mass spectrometry. The
Orbitrap Eclipse is designed to address todays needs in proteomic analysis. The instrument excels at deep
sequencing and quantitative proteomics as well as accurate characterization of post translational
modifications. The current mass spectrometers in the Core lack fast duty cycles and the advanced ion optics
that make detection of low abundance species routine on the Eclipse. The ability to unambiguously localize
post translational modifications (PTM) in peptides is a critical feature in any proteomic MS system and
the Eclipse is outstanding in this due to the availability of multiple fragmentation modes. Wayne State
University and the Henry Ford Health System are both strongly committed to this proposal as
evidenced by a combined $680,000 in new support for the instruments management, operation,
maintenance, informatics and usage. The technical expertise of Proteomics Core personnel as well
as the physical and administrative infrastructures are all in place and ready to immediately put this new
mass spectrometer to work on NIH-funded biomedical research projects. This instrument will provide
transformative technologies for advancing dozens of NIH-funded, ongoing projects, as well as catalyzing
new research directions for investigators at Wayne State University.

## Key facts

- **NIH application ID:** 10177090
- **Project number:** 1S10OD030484-01
- **Recipient organization:** WAYNE STATE UNIVERSITY
- **Principal Investigator:** PAUL M STEMMER
- **Activity code:** S10 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $1,289,466
- **Award type:** 1
- **Project period:** 2021-06-15 → 2022-06-14

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10177090

## Citation

> US National Institutes of Health, RePORTER application 10177090, Orbitrap Tribrid Mass Spectrometer for Wayne State Proteomics (1S10OD030484-01). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10177090. Licensed CC0.

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