# Cryo Transmission Electron Microscope for Cryo-EM Sample Optimization

> **NIH NIH S10** · CORNELL UNIVERSITY · 2021 · $1,051,430

## Abstract

PROJECT SUMMARY/ABSTRACT
 Cryo-electron microscopy (Cryo-EM) has heralded a resolution revolution in structural biology that has made
it possible for high-resolution structures to be determined for targets that have remained recalcitrant to
crystallization for decades. Cornell has invested in this future with the purchase of a high-end electron
microscope, the Arctica with a K3, allowing researchers an important valuable tool with which to reveal the atomic
details of their cryo-frozen biological samples. Unfortunately, we are scheduled to lose our dedicated low-end
screening microscope, the Morgagni, sometime in 2021 or possibly 2022. In analyzing our current workflow and
needs of the structural biology community at Cornell, we have concluded that the optimal replacement for the
Morgagni is a F200Ci microscope capable of both negative stain data collection and cryo-EM imaging.
 Acquiring such a microscope will improve overall throughput for Cornell scientists and the NIH-funded
investigators’ research programs, maximizing the utility of our highest-resolution and most in-demand
microscopes which are equipped with accessories crucial for high-resolution data collection: a the K3 direct
detector and a BioQuantum Energy Filter. This application requests funds to purchase a versatile cryo-electron
microscope ideally capable of negative stain imaging, cryo-sample screening, and micro-electron diffraction
(microED). It will serve NIH-funded faculty in five departments across Cornell, all invested in pursing the structure
of biomedically relevant and important biological systems related to human health, including membrane
receptors, membrane trafficking, nucleic-acid complexes involved in genome organization, and viral structure.

## Key facts

- **NIH application ID:** 10177173
- **Project number:** 1S10OD030470-01
- **Recipient organization:** CORNELL UNIVERSITY
- **Principal Investigator:** Elizabeth Kellogg
- **Activity code:** S10 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $1,051,430
- **Award type:** 1
- **Project period:** 2021-09-01 → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10177173

## Citation

> US National Institutes of Health, RePORTER application 10177173, Cryo Transmission Electron Microscope for Cryo-EM Sample Optimization (1S10OD030470-01). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10177173. Licensed CC0.

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