Cover Page: Descriptive Title: Combined single-molecule fluorescence confocal and dual-trap optical tweezer Proposed Project: 02/01/2021 and end date of 01/31/2022 Estimated Project Funding: Total Federal Funds Requested: $ 600,000 Total Non-Federal Funds: $ 0 Total Federal & Non-Federal Funds: $ 600,000 Program Income: $ 0 Major Users: Dr. Baron Chanda Dr. Eric Galburt Dr. Roberto Galletto Dr. Michael Greenberg Dr. Timothy Lohman Dr. Janice Robertson Dr. Andrea Soranno Dr. Meredith Jackrel Minor User Dr. Alex Holehouse Dr. Michael Vahey