Abstract Request for an Andor Dragonfly High Speed Confocal Platform. We are requesting the purchase of an Andor Dragonfly spinning disk confocal microscope (aka Dragonfly) for high- speed and high-resolution imaging of microscopic cellular features. This instrument incorporates high-speed fully scalable state-of-the-art confocal imaging capable of resolving sub-cellular protein targets at the cell membrane using total internal reflectance (TIRF), or deeper in the cell with SRRF-Stream and dSTORM super-resolution techniques. The Dragonfly uses Imaris for real-time graphics processing unit (GPU)-based image processing providing seamless integration with downstream image processing workflows and data analysis, platform interoperability, improved data integrity, audit compliance, and user productivity. The Dragonfly uses a proprietary multi-point confocal for high-speed and high- sensitivity imaging that is at least 10 times faster than conventional confocal technology. The unique illumination method enables even signal intensity across the field of view. The Dragonfly is the optimal solution to meet a broad array of rigorous live cell imaging needs that encompass analysis of behaving brain cells in ex vivo experiments examining calcium dynamics or neuroimmune cell function, cellular transport, synaptic vesicle trafficking, immune cell mobility and infiltration assays among others. As such, it will advance research into Aging, Dementia, Traumatic brain injury, and other emergent health concerns including COVID-19. The instrument will be placed in VA Puget Sound Health Care System (VA PSHCS) GRECC research space (Building 1 7th floor) and operated by trained staff. Primary users are BLR&D Merit review funded GRECC core staff: Drs. David Cook, Nicole Liachko, Brian Kraemer, Jose Garcia, and Chang-en Yu. Secondary users are MIRECC Investigators Jeffery Iliff, Dianne Lattemann, and James Meabon as well as GRECC core staff Erik Carlson and CDA2 recipient Rebecca Kow. Purchase of the Dragonfly is expected to increase the competitiveness of the overall VA PSHCS research program, significantly increase effectiveness and efficiency of confocal microscope imaging, and further the GRECC and MIRECC research missions by providing instrumentation necessary for robust cell and tissue based investigations into the molecular mechanisms of disease and aging.