# Accumulation, Storage, and Release of Sperm in the Oviduct

> **NIH NIH R01** · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · 2021 · $265,814

## Abstract

Project Summary
 Fertility depends on successful fertilization and early development, processes that occur in the oviduct. Common
therapies for human infertility, such as in vitro fertilization and intracytoplasmic sperm injection, are expensive and
increase risks of a variety of problems. More knowledge of how the oviduct interacts with sperm, the cumulus-oocyte
complex (COC) and the developing embryo may improve fertility and reduce the need for therapies. The oviduct serves as
a reservoir for sperm, after semen deposition and before fertilization. Binding to the oviduct maintains sperm viability and
suppresses motility. Sperm are released to move to the upper oviduct (ampulla) to fertilize oocytes. There are many gaps
in this model of sperm-oviduct interaction. Our studies have begun to fill some of these gaps. We have used a glycomic
approach to screen 377 glycans and found that all glycans with affinity for porcine sperm have either of two motifs,
sulfated Lewis X trisaccharide or branched 6-sialylated complex glycans. We have identified two candidate receptors for
both glycans on the sperm membrane, PKDREJ and ADAM5. Notably, mouse sperm deficient in PKDREJ and other
ADAMs do not accumulate beyond the utero-tubal junction, but it is not known if this is due to a problem in
binding and retention in the oviduct. Remarkably, if these glycans are immobilized on beads, they can extend sperm
lifespan, much like binding to oviduct cells prolongs the lifespan of sperm. Finally, we found that cumulus-oocyte
complexes (COCs) secrete molecules that signal sperm release from the lower oviduct so they can move toward the site of
fertilization. The Specific Aims will provide a mechanistic understanding of how sperm bind the oviduct, how binding
prolongs sperm lifespan and how sperm are released from the oviduct by the COC. Aim 1: To determine the function of
PKDREJ and ADAM5 in sperm by blocking each protein and mutating each gene in swine. Sperm from pigs that
are deficient in each of these proteins will be examined to determine if their ability to bind oviduct cells and their fertility
is affected. Aim 2. To determine if sperm binding to immobilized oviduct glycans suppresses ROS production,
conserves ATP, and stabilizes the plasma membrane, which extends sperm lifespan and fertility. Sperm bound to
immobilized glycans will be examined to ascertain what changes adhesion induces. The second group of experiments will
determine which changes are sufficient to prolong lifespan by adding agents that scavenge ROS and stabilize the plasma
membrane. Aim 3. To determine how the cumulus-oocyte complex signals release of sperm from oviduct cells and
glycans. We will examine whether progesterone, prostaglandins, or proteins that may be secreted from COCs might
induce porcine sperm release from oviduct cells and immobilized glycans. The completion of these Specific Aims will
elucidate how sperm bind to the oviduct, are stored in the oviduct and are released in resp...

## Key facts

- **NIH application ID:** 10179435
- **Project number:** 5R01HD095841-04
- **Recipient organization:** UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
- **Principal Investigator:** DAVID Joel MILLER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $265,814
- **Award type:** 5
- **Project period:** 2018-09-04 → 2023-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10179435

## Citation

> US National Institutes of Health, RePORTER application 10179435, Accumulation, Storage, and Release of Sperm in the Oviduct (5R01HD095841-04). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10179435. Licensed CC0.

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