In this ShEEP grant, we request funds to purchase a 4th Generation 3D-Bioplotter Manufacturer Series manufactured by EnvisionTEC, Inc. for research applications at VA Loma Linda Healthcare System. 3D printing is based on the principle of layered manufacturing, in which materials are overlapped layer by layer to quickly fabricate components with any complex shape by accurately accumulating material using solid modeling according to a computer-aided design model or computed tomography scan under computer control. The preparation of organ models, rapid manufacturing of personalized scaffolds, and direct printing at the defect site can be achieved by 3D printing technology based on imaging data. 3D printers are increasingly being used for a number of pre-clinical investigations including research on personalized manufacturing of permanent non-bioactive implants, fabricating local bioactive and biodegradable scaffolds and directly printing cells and tissues for regeneration studies. The 3D-Bioplotter Manufacturer Series from EnvisionTEC was designed as a tool for advanced tissue engineering and also for use in a production environment. It is the most versatile and advanced of the 3D-Bioplotter options and is capable of utilizing up to 5 materials in a build as well as UV curing capabilities and both heated and cooled platform options. It has a modular head design that allows users to add new features to existing machines. It is designed for use in a sterile environment within a biosafety cabinet. This new instrument will be used by a number of investigators at the VA Loma Linda Healthcare System (VALLHCS) to support research projects funded by VA merit review, NIH and DOD grants. The 3D-Bioplotter printing head modalities available in the Manufacturer Series system will allow 3D printing of materials for applications that include bone regeneration, drug release, cell/organ printing and soft tissue fabrication. A number of VA, DOD and NIH funded investigators at this VA Medical Center including Drs. Mohan, Lau, Rundle, Dong, Li, Venezia and Xing will benefit from being able to use this equipment to perform studies related to their funded grant applications. Currently, no advanced 3D-Bioplotter system is available for research use at VALLHCS. The requested instrument will greatly enhance experimental capabilities of our translational research that are relevant to the pathogenesis and treatment of osteoporosis, osteoarthritis and hearing loss, diseases that are target areas of the VA research mission.