# Response of the Bacterial Metalloproteome to Environmental Conditions

> **NIH NIH R01** · WOODS HOLE OCEANOGRAPHIC INSTITUTION · 2021 · $465,042

## Abstract

Response of the Bacterial Metalloproteome to Environmental Conditions
Abstract
 While many life forms are adapted to a narrow range of environmental redox conditions, opportunistic
pathogenic bacteria can be highly adaptable to varying environmental conditions. Large changes in oxygen
and nutrient availability require a complex suite of sensing, regulation, and biochemical choices in order to
allow and maintain cellular growth. Nutritional immunity strategies are important in pathogen-host relationships,
where iron and zinc are competed for by pathogen and host. Yet less is known about interactions for scarcer
important micronutrients such as cobalt and vitamin B12. This project will examine the response of the
Pseudomonas aeruginosa proteome and metalloproteome to varying O2 and metal environmental conditions.
Metalloproteomic approaches allow an assessment of the entire soluble metalloprotein pool for each metal
simultaneously, answering questions about the deployment of each metal within the metallome, and the
interaction between them through metalloenzyme substitutions under varying environmental conditions or
genetic manipulations. Given P. aeruginosa's genetic investment in multiple B12-requiring enzymes and their
Fe and Zn metalloenzyme substitutes, there is significant room for study on how it manages its metalloenzyme
complement and metal homeostasis across redox transitions. This proposal will examine how oxygen is a
master controller of metallome composition for P. aeruginosa, impacting the proteome and its metalloenzyme
composition. The acquisition of metals and B12 and production of metallophore ligands under oxygen and metal
availability conditions will be examined. Major metalloenyzmes will be quantified on an absolute basis to
understand the stoichiometric costs for their production across the aerobic to anaerobic gradient. The expected
outcome of this research is a detailed understanding of how the metallome of a major opportunistic pathogen is
influenced by environmental conditions, which could lead to development of treatment therapies.

## Key facts

- **NIH application ID:** 10180988
- **Project number:** 5R01GM135709-02
- **Recipient organization:** WOODS HOLE OCEANOGRAPHIC INSTITUTION
- **Principal Investigator:** Makoto Saito
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $465,042
- **Award type:** 5
- **Project period:** 2020-06-10 → 2024-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10180988

## Citation

> US National Institutes of Health, RePORTER application 10180988, Response of the Bacterial Metalloproteome to Environmental Conditions (5R01GM135709-02). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10180988. Licensed CC0.

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