# Neural activity-dependent modulation of cortical microvascular restoration

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA-IRVINE · 2021 · $565,233

## Abstract

Project Summary / Abstract
Blood vessels, from arteries to capillaries to venules and then to veins, contribute to fundamental physiological
processes. However, the vascular responses for repair and restoration of microvascular networks after cortical
brain injury are poorly understood, including how neuronal activity influences these processes. A major barrier
to research is the poor accessibility of micro-vessels in the brain and associated technical difficulties. To
overcome this barrier, we propose to use innovative imaging technologies that we have co-developed to
investigate micro-vessel formation and re-growth in response to focal cortical injury. We have used light-weight
head-mounted, miniaturized microscopes (“miniscopes”) to dynamically image the vasculature and associated
cells with high spatial and temporal resolution. We will use cortical injury models by applying a controlled
moderate impact to the mouse motor cortex. Combining in vivo longitudinal miniscope and 2-photon imaging,
histological “vessel painting” and perfusion-weighted magnetic resonance imaging (PWI MRI), we aim to achieve
a deeper understanding of microvascular restoration following cortical injury. We will apply targeted optogenetic
stimulation of excitatory neurons and specific inhibitory neurons to modulate microvascular repair in early and
late phases of vessel re-growth. Our guiding hypothesis is that microvascular restoration and remodeling after
cortical injury are regulated by vascularization sequences and cellular processes that are similarly observed in
normal vasculogenesis during central nervous system development. In Aim 1, we will identify the time course
and spatial pattern of vascular regrowth, and blood flow dynamics after focal cortical injury. Vascular networks
and blood flow are visualized with fluorescent-labeled dextrans for in vivo imaging for quantitative
measurements. In Aim 2, we will determine the role of endothelial cells in new blood vessel sprouting and the
establishment of functional microvascular by imaging Tie2-Cre reporter mice during the first two weeks post-
injury. We will also examine the influence of astrocytes and pericytes in vascular re-growth. In Aim 3, we will
test the hypothesis that optogenetic stimulation of specific neuron types in a temporally controlled manner
facilitates and enhances microvasculature restoration for post-injury repair. We will also examine if and how
targeted modulation of neural activities modulate Wnt/ß-catenin and VEGF signaling mechanisms that are critical
for micro-vessel re-growth. Behavioral testing will assess the outcomes of the optogenetic treatment. We have
strong preliminary data that supports the premise for the proposed research for all aims. The proposed research
will advance our understanding of the cellular and molecular mechanisms underlying cortical microvascular
restoration and how neural stimulation enhances vascular network formation.

## Key facts

- **NIH application ID:** 10181904
- **Project number:** 1R01NS121246-01
- **Recipient organization:** UNIVERSITY OF CALIFORNIA-IRVINE
- **Principal Investigator:** ANDRE OBENAUS
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $565,233
- **Award type:** 1
- **Project period:** 2021-03-15 → 2025-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10181904

## Citation

> US National Institutes of Health, RePORTER application 10181904, Neural activity-dependent modulation of cortical microvascular restoration (1R01NS121246-01). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10181904. Licensed CC0.

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