# Tissue Culture Core - Core A

> **NIH NIH P01** · UT SOUTHWESTERN MEDICAL CENTER · 2021 · $993,015

## Abstract

Core ATissue Culture Core will be responsible for providing investigators with cultured cells,
monoclonal antibodies, recombinant proteins, and recombinant adeno-associated viruses. The Core will be
directed by Dr. Joseph Goldstein, who has served as its Principal Investigator for the past 40 years. The
technical work in the Core is carried out by five experienced technicians, one of whom (Lisa Beatty) has been
in charge of this facility for the past 28 years. The physical facilities of the Core consists of three suites of rooms
that are used solely for tissue culture. The facility is equipped with 40 incubators, 14 inverted microscopes, 1
stereo microscope, 16 sterile work areas (hoods), 2 roller bottle apparatuses, 5 refrigerated incubator shakers,
3 table-top refrigerated centrifuges, 11 refrigerators, and 7 liquid nitrogen freezers for storage of cell lines.
 The success of the entire Program Project Grant depends on the smooth operation of this Core. Over
the past 40 years, we have developed considerable experience in maintaining quality control and in growing
multiple cell lines, including over 1100 different primary human fibroblast cell strains, derived from skin biopsies
from normal subjects as well as from patients with metabolic disorders. Since 1984, tens of thousands of
transfection experiments have been carried out in which we have introduced into various cell lines (e.g., HEK-
293, CHO, and SV589 cells) multiple plasmid constructs containing either the protein-coding region or the
promoter/enhancer region of multiple genes. From these transfections, more than 3000 stable and permanent
cell lines have been clonally established and frozen away in multiple aliquots.
 In addition to maintenance of stock cell lines and preparation of cultured cells for experiments, the Core
is involved in the following activities: 1) Generation and maintenance of mouse and rabbit hybridoma cell lines
and production of monoclonal antibodies from culture medium; 2) Purification by Protein A-Sepharose
chromatography of more than 25 different polyclonal rabbit antibodies directed against multiple proteins; 3)
Growth of large volumes of suspension-culture cells that allow efficient transfection of cDNAs and production
of their encoded proteins; 4) Isolating, maintaining, and freezing away cloned cell lines that have been
transfected with mutated versions of various cDNA and promoter/enhancer constructs; 5) Maintenance of
insect cells in suspension culture for production of recombinant proteins by infecting these cells with
recombinant baculoviruses encoding cloned cDNAs; 6) Production of recombinant adeno-associated viruses
and lentiviruses, which are used for evaluating the function of genes in cultured cells and in the liver after in
vivo injection; 7) Sending aliquots of monoclonal and polyclonal antibodies and cell lines to hundreds of
investigators who request them.
C/PPG 2015 – Core A – 30-line Summary

## Key facts

- **NIH application ID:** 10183286
- **Project number:** 5P01HL020948-45
- **Recipient organization:** UT SOUTHWESTERN MEDICAL CENTER
- **Principal Investigator:** JOSEPH L GOLDSTEIN
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $993,015
- **Award type:** 5
- **Project period:** 1997-07-15 → 2022-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10183286

## Citation

> US National Institutes of Health, RePORTER application 10183286, Tissue Culture Core - Core A (5P01HL020948-45). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10183286. Licensed CC0.

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