# ADRD Induced pluripotent stem cell/organoid core

> **NIH NIH P01** · BUCK INSTITUTE FOR RESEARCH ON AGING · 2021 · $339,832

## Abstract

PROJECT SUMMARY
The goals of the Program Project are to understand the causes and consequences of cellular senescence as a
driver of age-related neurodegeneration, determine new targets and mechanisms by which senescent cells drive
disease, and identify new targets for interventions that alter disease onset and/or progression. The Alzheimer’s
disease and related dementias (ADRD) induced pluripotent stem cell (iPSC)/organoid core, led by Drs.
Ellerby and Tracy, will provide expertise in generating iPSC-derived neurons, astrocytes, microglia, co-cultures
and brain organoids to understand cellular senescence as a driver of age-related neurodegeneration. It will
provide a suite of iPSC cellular models to all three projects and validate all the derived cell types, co-cultures
and brain organoids. The Core will provide essential infrastructure, training and support for all scientists
participating in the PPG. In addition, it will provide everything needed to culture, conduct electrophysiological
recordings, and image cells. The Core directors have experience in all of these cell manipulations and will
approve all Alzheimer’s-related disease (ARD) and control iPSC models for the facility, including live and frozen
cell cultures from commercial and academic sources, to ensure reproducibility, authentication, testing for
mycoplasma, and other cell line maintenance and validation tasks. These Core directors will approve all
Alzheimer’s disease related and control iPSC models for the facility, including live and frozen cell cultures from
commercial and academic sources, to ensure reproducibility, authentication, testing for mycoplasma, and other
cell line maintenance and validation tasks. The Core will train students and postdoctoral fellows and oversee
any research involving iPSCs that require ESCRO approvals, ethics training and MTAs. To accomplish these
goals, five specific aims of the ‘ADRD iPSC/Organoid Core’ are described. These goals are organized around
the cell type or technology that is needed to address the research questions described in the three projects in
an efficient and optimized approach. The Specific Aims of this Core are: Specific Aim 1 – Generate a bank of
iPSCs derived from the indicated models; Specific Aim 2 – Microglia. Generate microglia from iPSCs derived
from the models (Projects 1, 2 and 3); Specific Aim 3 – Astrocytes. Generate astrocytes from iPSCs derived from
the models (Projects 1 and 3); Specific Aim 4 – Cortical Neurons. Generate cortical neurons (excitatory and
inhibitory) from iPSCs derived from the models (Projects 1, 2 and 3); Specific Aim 5 – Cerebral Organoids and
Co-cultures. Provide cerebral organoids (Projects 1 and 3); Specific Aim 6 – Electrophysiology and Imaging.
We will perform electrophysiological recordings for Projects 1, 2 and 3 in both human cellular models and mouse
models. These aims will provide models and technology that will enhance our knowledge of cell type specificity
and interactions in AD cel...

## Key facts

- **NIH application ID:** 10187409
- **Project number:** 1P01AG066591-01A1
- **Recipient organization:** BUCK INSTITUTE FOR RESEARCH ON AGING
- **Principal Investigator:** Lisa M Ellerby
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $339,832
- **Award type:** 1
- **Project period:** 2021-09-30 → 2026-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10187409

## Citation

> US National Institutes of Health, RePORTER application 10187409, ADRD Induced pluripotent stem cell/organoid core (1P01AG066591-01A1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10187409. Licensed CC0.

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