# The Mouse Blood Center Core

> **NIH NIH P01** · UNIVERSITY OF VIRGINIA · 2021 · $417,881

## Abstract

Project Summary
Background: RBC alloimmunization is a clinically significant problem in transfusion and pregnancy settings
alike. Given the number of variables involved in human studies, the projects in this PPG have taken the
approach of using reductionist murine models to generate a better overall understanding of factors
influencing RBC alloimmunization. This proposed Mouse Blood Center Core (Core B) will support the 4 PPG
projects by providing leukoreduced, quality controlled, transgenic RBCs for transfusion. Further, this Core will
serve as a central immunohematology reference Laboratory for alloantibody testing. In addition to optimizing
resource utilization and efficiency, the products and services provided by this Core will allow for more direct
comparison of results between sites and projects.
Innovation: The mouse donor strains proposed for use in these studies, generated and characterized by
PPG investigators, allow for isolation of the effects of single variable changes on alloimmunization. The HOD
donors have a triple fusion protein on their RBCs, composed of hen egg lysozyme, ovalbumin, and the
human Duffyb antigen. The KEL donors have the entire human KEL glycoprotein expressed in an RBC
specific manner, with K1 as well as K2 donors being used in this proposal. Antibodies generated by wild type
recipient mice transfused with transgenic HOD, K1, or K2 RBCs are clinically significant, resulting in
premature RBC clearance, hemolytic transfusion reactions, and hemolytic disease of the fetus and newborn
(HDFN).
Specific Aim 1: To breed and maintain transgenic mice expressing model or human blood group
antigens on their RBCs.
Specific Aim 2: To collect, process, perform quality control measures, and distribute donor RBCs
from these transgenic animals to all 4 PPG sites.
Specific Aim 3: To serve as an immunohematology reference laboratory for antibody detection.

## Key facts

- **NIH application ID:** 10192791
- **Project number:** 5P01HL132819-05
- **Recipient organization:** UNIVERSITY OF VIRGINIA
- **Principal Investigator:** JEANNE E HENDRICKSON
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $417,881
- **Award type:** 5
- **Project period:** 2017-09-01 → 2023-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10192791

## Citation

> US National Institutes of Health, RePORTER application 10192791, The Mouse Blood Center Core (5P01HL132819-05). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10192791. Licensed CC0.

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