# The ubiquitin proteasome system in ER quality control

> **NIH NIH R01** · STANFORD UNIVERSITY · 2020 · $18,383

## Abstract

Project Summary/Abstract
Folding and assembly of proteins synthesized in the endoplasmic reticulum is
closely monitored by a quality control apparatus that diverts folding-defective
products to the cytosol to be degraded by the ubiquitin-proteasome system by a
process known as endoplasmic reticulum-associated degradation (ERAD). The
long-term goal of this project is to elucidate the mechanisms by which ERAD
recognizes and destroys its targets. In the previous funding period we
successfully implemented a large scale functional genomic analysis of the
mammalian ERAD system that allowed us to perform unbiased analysis of
substrate-selective ERAD in mammals. These data led to critical discoveries
about the mechanisms of substrate triage and delivery to the HRD1
dislocon/ligase and the role of unconventional ubiquitin conjugation in coupling
dislocation to degradation. The studies proposed in the present application
harness state-of-the-art technologies that extend these discoveries and if
successful will bring about a detailed molecular-level understanding of triage and
quality control in the early secretory pathway.

## Key facts

- **NIH application ID:** 10193992
- **Project number:** 3R01GM074874-14S1
- **Recipient organization:** STANFORD UNIVERSITY
- **Principal Investigator:** RON R KOPITO
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $18,383
- **Award type:** 3
- **Project period:** 2006-05-01 → 2023-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10193992

## Citation

> US National Institutes of Health, RePORTER application 10193992, The ubiquitin proteasome system in ER quality control (3R01GM074874-14S1). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10193992. Licensed CC0.

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