# Biochemistry of Pre-mRNA Splicing

> **NIH NIH R37** · COLD SPRING HARBOR LABORATORY · 2021 · $806,400

## Abstract

The broad objective of this project is to elucidate key mechanistic and regulatory features of human pre-mRNA splicing, and its functional links to nonsense-mediated mRNA decay. Some of these mechanistic insights will be leveraged to develop targeted antisense oligonucleotide therapeutics, which can be used in the long term for clinical applications in precision medicine.

The proposed experiments involve a broad range of experimental and computational approaches, including cell-free and cell-based assays, high-throughput RNA-sequencing and quantitative proteomics, bioinformatics, transgenic mouse models, and antisense pharmacology. The anticipated findings will facilitate therapeutic antisense oligonucleotide design for various diseases, besides expanding our fundamental knowledge of basic post-transcriptional mechanisms and regulation.

## Key facts

- **NIH application ID:** 10197935
- **Project number:** 5R37GM042699-31
- **Recipient organization:** COLD SPRING HARBOR LABORATORY
- **Principal Investigator:** Adrian R Krainer
- **Activity code:** R37 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $806,400
- **Award type:** 5
- **Project period:** 1989-07-01 → 2023-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10197935

## Citation

> US National Institutes of Health, RePORTER application 10197935, Biochemistry of Pre-mRNA Splicing (5R37GM042699-31). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10197935. Licensed CC0.

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