# Identification of novel regulators of liver regeneration using single-cell approaches

> **NIH NIH F32** · BRIGHAM AND WOMEN'S HOSPITAL · 2021 · $70,458

## Abstract

Project Summary
The liver is an essential organ and the only solid organ capable of regeneration. Despite this, liver regeneration
is compromised in patients with advanced liver disease, and so understanding the mechanisms of liver
regeneration has significant implications for regenerative medicine. Zebrafish have emerged as a powerful
experimental system for studying regeneration, and there are several models of liver regeneration in zebrafish.
I propose to thoroughly characterize the process of liver regeneration in zebrafish using a combination of
classical tools and new techniques. I will use single-cell sequencing to identify more markers for described cell
types, discover rare or transient cell populations, reconstruct lineage transitions, and elucidate the transcriptional
responses to injury. I will validate these data by multicolor fluorescent in situ hybridization to gain spatial insight
into gene expression. I will utilize a new technique to combine single-cell sequencing data with genetic lineage
tracing to reveal the cellular basis of liver regeneration after severe liver injury. These findings will be validated
with classical lineage tracing techniques in order to study the signaling pathways required for liver progenitor
cells during regeneration. These data will further our understanding of the molecular and cellular basis of liver
regeneration in zebrafish, aiding our ability to develop therapies to enhance liver regeneration in humans.

## Key facts

- **NIH application ID:** 10198653
- **Project number:** 5F32AA027135-03
- **Recipient organization:** BRIGHAM AND WOMEN'S HOSPITAL
- **Principal Investigator:** Isaac Max Oderberg
- **Activity code:** F32 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $70,458
- **Award type:** 5
- **Project period:** 2019-07-01 → 2022-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10198653

## Citation

> US National Institutes of Health, RePORTER application 10198653, Identification of novel regulators of liver regeneration using single-cell approaches (5F32AA027135-03). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10198653. Licensed CC0.

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