# Ceramide-mediated pathology in periodontitis

> **NIH NIH R03** · NOVA SOUTHEASTERN UNIVERSITY · 2020 · $89,765

## Abstract

ABSTRACT
Ceramides in the cytoplasm of host cells are attracting interests as second messengers in a variety of cell events,
especially pro-inflammatory response. However, membrane structures of most bacteria, as distinct from that of
vertebrate, do not contain ceramide, indicating that production of ceramides by bacteria is rare. Nonetheless,
phosphoglycerol dihydroceramide (PGDHC), but not LPS, produced by Porphyromonas gingivalis is found
abundantly in the human periodontitis lesion. Contrast to the membrane impermeable ceramides derived from
vertebrates, we reported that PGDHC penetrates the cellular membrane of host osteoclast precursor (OCP) and
promotes cell fusion required for osteoclastogenesis by acting on a non-muscle myosin IIA (Myh9). Myh9 is an
intracellular down-regulatory factor for cell fusion event in osteoclastogenesis. In contrast, it was also
demonstrated that host ceramides directly act on cathepsin B to activate its catalytic activity. Furthermore,
cathepsin B promotes RANKL-mediated osteoclastogenesis via temporally controlled proteolysis of Myh9 for
initiation of cell fusion between OCPs. Our preliminary data demonstrated that PGDHC-mediated upregulation
of osteoclastogenesis is attenuated by the chemical inhibitor for cathepsin B, indicating that cathepsin B is also
engaged in the PGDHC-mediated osteoclastogenesis. Importantly, the intracellular levels of ceramides, either
derived from host or bacteria, are regulated by ceramidases in the physiological context. Dysregulated function
of acid ceramidase is implicated in the pathogenesis of Farber Disease and other medical complications. We
discovered and published that expression of acid ceramidase in patients with periodontitis is diminished.
Nonetheless, our knowledges about the ability of acid ceramidase to regulate the level of PGDHC as well as
molecular mechanisms underlying PGDHC-mediated osteoclastogenesis are not yet understood well. Thus, we
hypothesized that diminished aCDase activity in periodontal lesions retains the pathogenic property of tissue-
penetrating PGDHC which, in turn, elevates the osteoclastogenesis via novel Cathepsin B/Myh9 axis in the bone
resorption lesion of periodontitis. The Aim 1 is designed to test the protective role of acid ceramidase against
PGDHC-mediated osteoclastognesis in a mouse model of periodontitis. We will evaluate the effect of
endogenous acid ceramidase as well as exogenously administered recombinant acid ceramidase on neutralizing
the PGDHC’s pathogenic activity. In Aim 2, using loss- and gain-of-function approaches, we will evaluate the
engagement of cathepsin B in the PGDHC-mediated OCP fusion via Myh9 proteolysis. The proposed research
project will, for the first time, elucidate the molecular mechanism underlying the pathogenically elevated
periodontal bone loss targeting the unique P. gingivalis ceramides that promote osteoclastogenesis by
modulating the cell fusion regulatory factor, Myh9, and investigate the effects o...

## Key facts

- **NIH application ID:** 10199552
- **Project number:** 3R03DE027153-02S1
- **Recipient organization:** NOVA SOUTHEASTERN UNIVERSITY
- **Principal Investigator:** Alexandru Movila
- **Activity code:** R03 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $89,765
- **Award type:** 3
- **Project period:** 2020-09-01 → 2021-09-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10199552

## Citation

> US National Institutes of Health, RePORTER application 10199552, Ceramide-mediated pathology in periodontitis (3R03DE027153-02S1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10199552. Licensed CC0.

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