# Characterizing proteasome-substrate interactions by mass spectrometry proteomics

> **NIH NIH R01** · TRUSTEES OF INDIANA UNIVERSITY · 2021 · $341,783

## Abstract

PROJECT SUMMARY
This application features a close collaboration between bioanalytical (Dr. Reilly, Indiana University) and bio-
chemistry (Dr. Matouschek, University of Texas, Austin) research groups aimed at improving our understanding
of the ubiquitin-proteasome protein degradation system. The investigators contribute complementary expertise
and experience in a variety of disciplines ranging from proteomics, protein bioinformatics, analytical chemistry
and instrumentation, to biochemistry, molecular biology and cell biology. At Indiana University, cross-linking
mass spectrometry will be combined with biochemical and genetic assays to characterize the interaction between
the proteasome and its substrates to provide candidates for novel substrate receptors on the proteasome. At
The University of Texas at Austin, the biological relevance of candidates will then be tested in biochemical pro-
teasome assays and genetic yeast experiments. These assays will define the contribution of newly identified
components to substrate recognition and degradation by the proteasome and elucidate the molecular and struc-
tural origin of substrate selection and specificity.
Overall, we believe that this proposal will result in significant advances in cross-linking methodology and in our
understanding of how the proteasome recognizes and digests substrates.

## Key facts

- **NIH application ID:** 10200097
- **Project number:** 5R01GM135264-02
- **Recipient organization:** TRUSTEES OF INDIANA UNIVERSITY
- **Principal Investigator:** DAVID E. CLEMMER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $341,783
- **Award type:** 5
- **Project period:** 2020-07-01 → 2025-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10200097

## Citation

> US National Institutes of Health, RePORTER application 10200097, Characterizing proteasome-substrate interactions by mass spectrometry proteomics (5R01GM135264-02). Retrieved via AI Analytics 2026-05-21 from https://api.ai-analytics.org/grant/nih/10200097. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*