# Design and Analysis of Human Gene Mapping Studies

> **NIH NIH R01** · UNIVERSITY OF MICHIGAN AT ANN ARBOR · 2021 · $489,299

## Abstract

The Human Genome Project and follow-on projects such as 1000 Genomes, GTEx, ENCODE, and TOPMed
provide powerful resources to identify genes that influence human health and disease and variability in
disease-related quantitative traits (QTs). Along with these resources have come increasingly efficient tools to
genotype, sequence, and annotate the genome, and to support computation across these data. These resources
and tools will be critical as we continue to explore the genetic basis of human disease and disease-related QTs.
 In this proposal, we describe statistical and computational problems that arise in human gene mapping,
with a particular focus on sequence analysis, genotype imputation, and quality control. We describe statistical
methods to address these problems and software tools and web services to facilitate their use. We will test
resulting methods, tools, and web services via computer simulation and analysis of data from complex trait
genetics studies in which we are involved. Specifically, we will:
(1) develop tools to detect and estimate DNA sample contamination that are agnostic to genetic ancestry;
(2) develop a test for Hardy-Weinberg equilibrium of sequence-based or imputed genotypes in the presence of
population structure and robust to sample contamination;
(3) enable more accurate variant filtering and genotype calling from DNA sequence data in the presence of
population structure and/or sample contamination;
(4) develop methods to detect sample contamination in RNA- and epigenomic sequence data;
(5) extend the Michigan Imputation Server (MIS) to increase power of a sequence-based association studies by
 supporting use of external controls from existing sequence data resources, augmenting an existing imputation
reference panel with the investigator's sequenced samples, and checking for contamination; and
(6) document, distribute, and support efficient software tools to support these methods.
 Under separate funding, we will apply the resulting methods to help understand the genetic basis of type 2
diabetes and related QTs, and of schizophrenia and bipolar disorder.
 Success in these aims will enable more rapid identification of variants that predispose to human disease
and account for variability in disease-related QTs, and has the potential to lead to new insights into basic
biology and disease etiology, identify novel therapies, improve targeting of therapies, assist in disease
classification, and support more accurate disease risk prediction. The modest cost of statistical and
computational methods development, and the impact of these methods across many studies, makes our
proposed research highly cost effective.

## Key facts

- **NIH application ID:** 10200112
- **Project number:** 5R01HG009976-04
- **Recipient organization:** UNIVERSITY OF MICHIGAN AT ANN ARBOR
- **Principal Investigator:** MICHAEL L BOEHNKE
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $489,299
- **Award type:** 5
- **Project period:** 2018-09-07 → 2023-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10200112

## Citation

> US National Institutes of Health, RePORTER application 10200112, Design and Analysis of Human Gene Mapping Studies (5R01HG009976-04). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10200112. Licensed CC0.

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