# Flow Cytometry

> **NIH NIH P30** · OREGON HEALTH & SCIENCE UNIVERSITY · 2021 · $49,947

## Abstract

FLOW CYTOMETRY SHARED RESOURCE
Director: Philip R Streeter, PhD
ABSTRACT / PROJECT SUMMARY
The Knight Flow Cytometry Shared Resource provides Knight Cancer Institute members with state-of-the-art
instrumentation, expertise, and infrastructure that fully support investigator needs for analytical flow cytometry
and cell sorting. In the cancer setting, flow cytometry offers investigators a means of studying cells derived from
diverse hematologic and solid cancers, non-cancerous cells from the microenvironments in which cancers
reside, and cells from non-cancerous regions of the body. Flow cytometry facilitates the investigation of cells
either at the population level or at the single cell level. In addition, the integrated use of cell isolation (by flow
cytometry; within the Flow Cytometry Shared Resource) and downstream assessment of isolated cells (by
proteomics or genomics; within the Proteomics Shared Resource and/or the Integrated Genomics Shared
Resource) provides Knight investigators both the structure and technical expertise to facilitate highly complex
interdisciplinary studies. The primary areas of focus for the Flow Cytometry Shared Resource staff are customer
service/support, continuous improvement of standard flow cytometric technologies, and the
introduction/establishment of new technologies or platforms to enable cutting-edge scientific investigation by
Knight members. The services and technologies offered thru the Flow Cytometry Shared Resource are broadly
supportive of Knight members, with nearly two-thirds of total Flow Cytometry Shared Resource usage by Knight
members. Strategic decisions that impact the facility are made by the Knight senior leadership and the Flow
Cytometry Shared Resource Advisory Committee. Support and services include: consultations, contributions to
grant applications, education, training, data analyses, analytical flow cytometry, and cell sorting. Further, Knight
investigators have the option of instrument self-use or operator-assisted use. The successful
introduction/establishment of new technologies has allowed the Flow Cytometry Shared Resource to rapidly
adapt to the changing needs of Knight members. By way of example, in 2012, a CyTOF instrument was
purchased for the Flow Cytometry Shared Resource. The CyTOF, a mass cytometer, incorporates cytometry
and mass spectroscopy, allowing investigators the potential to simultaneously analyze up to 34 parameters
associated with individual cells. The resource also obtained a Luminex 200, an instrument enabling multiplex
measurements of up to 100 analytes in solution. The technologies offered by the Flow Cytometry Shared
Resource are having a positive transformative impact on research conducted by many investigators within the
Knight. The establishment of new technologies has allowed the Flow Cytometry Shared Resource to have a
central role in the research successes of many Knight members. During the next grant cycle, the Flow Cytometry
Shared Resource will be ex...

## Key facts

- **NIH application ID:** 10205357
- **Project number:** 5P30CA069533-23
- **Recipient organization:** OREGON HEALTH & SCIENCE UNIVERSITY
- **Principal Investigator:** PHILIP R STREETER
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $49,947
- **Award type:** 5
- **Project period:** 1997-08-01 → 2022-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10205357

## Citation

> US National Institutes of Health, RePORTER application 10205357, Flow Cytometry (5P30CA069533-23). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10205357. Licensed CC0.

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