# Optically mapping tissue biomechanics during neural tube closure

> **NIH NIH K25** · UNIV OF MARYLAND, COLLEGE PARK · 2021 · $114,021

## Abstract

Project Summary
This project features the development of advanced photonic technology to attack a major unmet challenge in
developmental biology.
Embryonic morphogenesis results from a complex combination of gene expression, biochemical signaling, and
biomechanics. While methods to evaluate the first two are well established, our knowledge of biomechanics of
the embryo morphogenesis is poorly understood because of the lack of technical approaches. Elucidating the
biomechanics underlying morphogenesis is essential towards understanding the interplay between mechanical
regulation, gene expression and tissue patterning that drive embryogenesis, and will potentially lead to exciting
innovations in therapeutic strategies and diagnostics for developmental defects. Current technology for tissue
elasticity measurement is slow and invasive, thus cannot measure mechanical properties within living 3D
embryonic tissue in-situ. This project will develop an all-optical approach (line-scanning Brillouin microscopy,
LSBM) to fulfill this unmet need. LSBM allows rapid 3D mapping of the elasticity of embryonic tissue in-situ with
high-resolution, non-invasive, and non-contact manner.
After technology validation, I will use this technique to address an open question of the development field related
to the role of tissue biomechanics in the process of neural tube closure. The central hypothesis of this grant is
that the neural tube defect is related to the altered mechanical properties of tissue. Specifically, I will investigate
the role of cellular activities, such as apical constriction, in the stiffness change of tissue during different stages
of neurulation and specific genetic factors contribute to the abnormal changes in tissue stiffness.
This K25 award, through its training and research components, will provide me with the skills to create a strong
biological part in my future research, in which I will utilize the enabling technological capabilities to address the
important needs in developmental biology. The overall effort will hasten my transition to being an independent
investigator at the forefront of the interdisciplinary interface of technology development and biomedical research.

## Key facts

- **NIH application ID:** 10208917
- **Project number:** 5K25HD097288-03
- **Recipient organization:** UNIV OF MARYLAND, COLLEGE PARK
- **Principal Investigator:** Jitao Zhang
- **Activity code:** K25 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $114,021
- **Award type:** 5
- **Project period:** 2019-08-01 → 2024-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10208917

## Citation

> US National Institutes of Health, RePORTER application 10208917, Optically mapping tissue biomechanics during neural tube closure (5K25HD097288-03). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10208917. Licensed CC0.

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