# Axonal Transport of mRNA for Mitochondrial Proteins

> **NIH NIH R01** · BOSTON CHILDREN'S HOSPITAL · 2021 · $445,937

## Abstract

Neurons have more extended and complex shapes than any other cell and consequently face a
far greater challenge in distributing and maintaining mitochondria throughout their arbors.
Neurons can last a lifetime, but proteins turn over rapidly. Mitochondria, therefore, need
constant rejuvenation of their protein components no matter how far they are from the soma
where the genes for most mitochondrial proteins reside. Transport of mitochondria from soma to
periphery may be one means of rejuvenating the peripheral population, but mounting evidence
indicates that local protein synthesis in axons and dendrites may also supply mitochondrial
needs. This may be particularly true for proteins with very short half-lives; proteins that would
be unlikely to survive the long trip down an axon. One such protein is PINK1, whose half-life is
estimated to be on the order of a few minutes. Constant synthesis and degradation of PINK1 is
an essential feature of current models for PINK1 function in mitochondrial quality control.
Consistent with this model, we have found that blocking proteins synthesis selectively in axons
prevents the local activation of the PINK1/Parkin pathway for mitophagy and that PINK1 mRNA
is enriched in axons. The current proposal is based on these findings and also the observation
that PINK1 mRNA colocalizes with mitochondria in axons and dendrites and is present on
moving mitochondria. We have therefore hypothesized 1) the existence of a mechanism to
localize PINK1 mRNA, and potentially many other transcripts for mitochondrial proteins, to the
surface of the mitochondrion and 2) that mRNA for PINK1 is transported into axons and
dendrites by virtue of its association with mitochondria. We have therefore proposed to identify
the sequences within the PINK1 transcript that are required for its association with mitochondria,
to identify the protein factors that mediate that association, and to determine if the association is
required for the presence of the transcript in axons and for the local induction of PINK1-
dependent mitophagy. We further propose to determine whether a similar mechanism operates
for other proteins and is necessary for preserving mitochondrial and axonal health. Because
defects in mitochondrial transport and mitophagy are implicated in Parkinson's and other
neurodegenerative disorders, it is necessary to understand how a neuron can preserve
mitochondrial health in a vast arbor and whether the transport of mRNA on mitochondria is part
of that mechanism.

## Key facts

- **NIH application ID:** 10210451
- **Project number:** 5R01NS107490-04
- **Recipient organization:** BOSTON CHILDREN'S HOSPITAL
- **Principal Investigator:** Thomas L. Schwarz
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $445,937
- **Award type:** 5
- **Project period:** 2018-08-01 → 2023-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10210451

## Citation

> US National Institutes of Health, RePORTER application 10210451, Axonal Transport of mRNA for Mitochondrial Proteins (5R01NS107490-04). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10210451. Licensed CC0.

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