# Humanized Mouse Core

> **NIH NIH P01** · OREGON HEALTH & SCIENCE UNIVERSITY · 2021 · $267,495

## Abstract

CORE A PROJECT SUMMARY/ABSTRACT
The primary goal of this Core is to generate humanized mice that can be used to test HCMV latency and
reactivation as well as hematopoiesis using HCMV mutants generated in Projects 1-5. The long-term goal of
Core A is to generate huBLT mice to answer in vivo questions of HCMV latency, reactivation and
hematopoiesis based on in vitro CMV models proposed in the various studies embedded in the Program
Project. First, the Core will generate huBLT mice to analyze HCMV mutants generated in Projects 1-4 for their
ability to establish and maintain latency and reactivate following treatment with G-CSF and AMD-3100. Once
Projects 1-4 will have identified signaling pathways and/or secreted factors that are important for HCMV
latency and reactivation, Core A will validate them using chemical inhibitors, neutralizing antibodies,
recombinant HCMV expressing shRNAs and/or addition of cytokines/growth factors required for reactivation.
Second, the Core will analyze hematopoiesis in huBLT mice infected with WT HCMV (Project 5) or viral
mutants (Projects 1-4) and then will validate the results using chemical inhibitors, neutralizing antibodies,
recombinant HCMV expressing shRNAs and/or addition of cytokines/growth factors required for hematopoiesis.

## Key facts

- **NIH application ID:** 10216631
- **Project number:** 5P01AI127335-05
- **Recipient organization:** OREGON HEALTH & SCIENCE UNIVERSITY
- **Principal Investigator:** Patrizia Caposio
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $267,495
- **Award type:** 5
- **Project period:** 2017-08-15 → 2022-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10216631

## Citation

> US National Institutes of Health, RePORTER application 10216631, Humanized Mouse Core (5P01AI127335-05). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10216631. Licensed CC0.

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