# MHC Genetic Typing

> **NIH NIH U42** · UNIVERSITY OF PUERTO RICO MED SCIENCES · 2021 · $280,582

## Abstract

PROJECT SUMMARY/ABSTRACT:
The major goal of this application is to sustain the Specific Pathogen Free (SPF) program at the Caribbean
Primate Research Center's (CPRC) through support of operations, administration, veterinary care, virological
and MHC typing and by the improvement of our comprehensive genetic management plan. A unique feature of
CPRC animals in favoring a successful establishment and sustainable of SPF colony are that these animals are
naturally free of SIV and SRV-D, two of the four viruses to be tested for. Nevertheless, for safety and as required
for the SPF Program, we continue systematically testing for all four viruses (B virus, STLV, SIV and SRV-D). The
Program provides unadmixed Indian-origin rhesus monkeys with defined genetic and virological background.
The population genetic analyses revealed that the SPF colony has remained genetically homogenous over time
with sufficient amounts of heterozygosity and minimal stratification from its non-SPF founders. Genetic
variation will be maintained in the SPF colony using the genetic management strategies described in this
application. The genetic management approach will allow for enrichment of desired MHC markers in demand
for biomedical research while maintaining genetic diversity. This specific core is designed to continue our current
efforts to maintain a genetically diverse, MHC-defined SPF Indian-origin rhesus macaque colony for use in
AIDS-related research. In 2015, the KDL began its collaboration with the current U42 to design and improve the
SPF breeding colony to enhance the efficiency of colony maintenance as well as to maximize genetic
heterogeneity of the colony animals. Since then we have been constructing multigenerational SPF pedigrees at
the CPRC which can be traced back to founders in the Cayo Santiago island population.

## Key facts

- **NIH application ID:** 10217280
- **Project number:** 5U42OD021458-20
- **Recipient organization:** UNIVERSITY OF PUERTO RICO MED SCIENCES
- **Principal Investigator:** CARLOS A SARIOL
- **Activity code:** U42 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $280,582
- **Award type:** 5
- **Project period:** 2016-07-01 → 2024-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10217280

## Citation

> US National Institutes of Health, RePORTER application 10217280, MHC Genetic Typing (5U42OD021458-20). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10217280. Licensed CC0.

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