# Genome-Wide Regulation of the TATA Binding Protein

> **NIH NIH R01** · CORNELL UNIVERSITY · 2020 · $193,263

## Abstract

Project Summary/Abstract
The budding yeast Saccharomyces cerevisiae is used here as model system to understand genomic mechanisms
by which genes are regulated. Changes in the disposition of regulatory factors bound across the genome will be
monitored when the genome is reprogrammed. The high resolution ChIP-exo and other related assays will be
used to monitor genome-wide binding events at near single-bp resolution. Heat shock will be the primary
means by which the genome is rapidly reprogrammed since the immediacy of its effect largely ensures that the
responding events are a direct consequence of heat shock sensing. This contrasts with other slower
environmental sensing systems, where responses may include indirect effects. The general goal of this
proposal is to understand gene co-regulation. The work will focus primarily on two distinct classes of genes:
ribosomal protein genes (Aim 1), and heat shock induced genes (Aim 2). By defining which factors bind to
responding genes and examining the how relevant factors are positionally organized spatially and temporally,
and in the context of mutants that are defective in relevant functions, a deeper mechanistic understanding of
gene control is obtained. Since yeast is a model for general eukaryotic systems, including human, related
biological concepts and approaches developed in yeast will be applied to human cells (Aim 3). This will provide
a streamlined approach towards getting at critical questions in human gene regulation, many of which have
been opaque to concept development in the absence of a driving model system.

## Key facts

- **NIH application ID:** 10219015
- **Project number:** 7R01GM059055-21
- **Recipient organization:** CORNELL UNIVERSITY
- **Principal Investigator:** B FRANKLIN PUGH
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $193,263
- **Award type:** 7
- **Project period:** 2000-02-01 → 2023-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10219015

## Citation

> US National Institutes of Health, RePORTER application 10219015, Genome-Wide Regulation of the TATA Binding Protein (7R01GM059055-21). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10219015. Licensed CC0.

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