# Interactions Discovery Core

> **NIH NIH P50** · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · 2021 · $301,972

## Abstract

Interactions Discovery Core Abstract
The Interactions Discovery Core (IDC) will continue to support interactive projects focusing on cellular protein
complexes mediating early events in HIV-1 infection. The projects carried out by the IDC will involve multiple
components of the Center as well as outside investigators. Aim 1 proposes to identify cellular proteins binding
specifically to HIV-1 capsid sites under positive selection. These highly collaborative experiments will be
supported by the Protein Core and Project 3, which will provide recombinant CA and/or soluble oligomeric CA
assemblies, and performed in collaboration with the HIV Virology Core, which will validate virologically high
priority hits. The virologically relevant targets will subsequently enter the PCHPI structure determination
pipeline. Aim 2 proposes to identify CPSF6 interacting proteins that mediate HIV-1 integration into genes; the
candidate functional CPSF6 partners will be investigated further in A. Engelman's lab. Aim 3 proposes to
identify novel HIV Vpr targets in DNA repair and replication pathways, and in particular those responsible for
the induction of DNA damage checkpoint by Vpr. The latter experiments will be performed in collaboration
with Project 6, which will validate the hits, and thereby will continue to provide the identity of new Vpr
complexes with cellular proteins for mechanistic and structural studies in the PCHPI. Thus, overall, the IDC is
completely integrated and central to many aspect of science being pursued at PCHPI.

## Key facts

- **NIH application ID:** 10219092
- **Project number:** 5P50AI150481-15
- **Recipient organization:** UNIVERSITY OF PITTSBURGH AT PITTSBURGH
- **Principal Investigator:** Jacek Skowronski
- **Activity code:** P50 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $301,972
- **Award type:** 5
- **Project period:** 2007-08-27 → 2023-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10219092

## Citation

> US National Institutes of Health, RePORTER application 10219092, Interactions Discovery Core (5P50AI150481-15). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10219092. Licensed CC0.

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