# Project 2:  Capsid dynamics and interactions with host factors

> **NIH NIH P50** · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · 2021 · $301,972

## Abstract

P2. Abstract
In this project, we will test our hypothesis, established through extensive studies at the PCHPI using multiple
techniques, that dynamics are an important factor determining capsid assembly, maturation, and interactions
with host proteins, such as TRIM5α. Specifically, we will i) determine the role of conformational dynamics in CA
assemblies; ii) determine the capsid/TRIM5α dynamics and delineate the intermolecular interfaces at atomic
resolution; and iii) determine the dynamics of capsid/CypA and structural perturbations induced by CypA
binding to CA assemblies from different strains exhibiting differences in capsid stability. To accomplish the
specific aims of this project, we will employ a multi-pronged approach including MAS NMR, cryo-EM, MD
simulations, as well as virology and molecular biology approaches. We will combine the complementary expertise
of the PCHPI investigators and collaborators and work in close synergy to tackle the multi-disciplinary questions
concerning the role of dynamics in the HIV-1 infection cycle.

## Key facts

- **NIH application ID:** 10219100
- **Project number:** 5P50AI150481-15
- **Recipient organization:** UNIVERSITY OF PITTSBURGH AT PITTSBURGH
- **Principal Investigator:** Tatyana Polenova
- **Activity code:** P50 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $301,972
- **Award type:** 5
- **Project period:** 2007-08-27 → 2023-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10219100

## Citation

> US National Institutes of Health, RePORTER application 10219100, Project 2:  Capsid dynamics and interactions with host factors (5P50AI150481-15). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10219100. Licensed CC0.

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