# Analysis of Migration Associated Treatment Resistance in Glioblastoma to Identify Novel Therapeutics

> **NIH NIH P20** · UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR · 2021 · $210,318

## Abstract

The migration and invasion of malignant Glioblastoma (GBM) cells into normal brain parenchyma is
fundamentally different than other biological conditions because the biophysical properties of the brain
microenvironment are differ from other tissues. These microenvironmental processes contribute to
chemotherapy resistance. New microfluidic ex vivo devices, that simulate the microenvironment of tumor cells
in the brain, will be used to evaluate the roll of active cell migration through confined spaces in developing
treatment resistance by (1) isolating and enriching clonal populations resistant to temozolomide and able to
migrate, and (2) testing the roll of candidate genes for chemotherapy resistance. Cell cultures and patient
derived stem cell lines will be enriched for migrating and treatment resistance cells to establish clonal
populations from heterogeneous cultures. These enrichment techniques will serve as future substrates for
treatment resistance models. By preliminary and reported data, ABCG2 is involved in GBM cell migration and
treatment resistance. Using the microfluidic channels, the expression pattern and role of ABCG2 will be
evaluated for ABCG2 necessity (expression knockdown and inhibitor) and sufficiency (overexpression). We
will analyze the expression pattern of ABCG2 to migration, pre-migration, during active migration and post-
migration. These results will add to our knowledge of GBM cell migration and establish a platform to test new
agents to sensitize migrating cells to conventional treatments.

## Key facts

- **NIH application ID:** 10219284
- **Project number:** 5P20GM103639-09
- **Recipient organization:** UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
- **Principal Investigator:** James Battiste
- **Activity code:** P20 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $210,318
- **Award type:** 5
- **Project period:** 2012-09-05 → 2023-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10219284

## Citation

> US National Institutes of Health, RePORTER application 10219284, Analysis of Migration Associated Treatment Resistance in Glioblastoma to Identify Novel Therapeutics (5P20GM103639-09). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10219284. Licensed CC0.

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