# Investigating long noncoding RNAs as regulators of the p53 pathway functions.

> **NIH NIH R35** · SLOAN-KETTERING INST CAN RESEARCH · 2021 · $449,000

## Abstract

ABSTRACT
 Long noncoding RNAs (lncRNAs) have emerged as pervasive features of the mammalian
genome with recent annotations identifying >50,000 lncRNAs in humans. Despite their prevalence,
few of these transcripts have been functionally characterized. While multiple lines of evidence point to
roles for lncRNAs in chromatin and transcription regulation, rigorous definition of the physiological
effects of these molecules remains rare. However, our recent work has identified an evolutionarily
conserved physiological role for a lncRNA that binds p53, further demonstrating that lncRNAs
contribute to critically important cellular processes. The realization that lncRNAs can bind p53 and
modulate p53 function establishes a new paradigm for information input onto p53, one in which
alterations in the transcriptional landscape of cells may inform global reprograming of p53
physiological responses.
 In this proposal, we will systematically examine the role of p53-bound lncRNAs in cellular
stress responses. We will leverage recent technical advances in genome-wide transcription
engineering by CRISPR to systematically modulate the expression of p53-bound lncRNA expression
and examine the the physiological consequences.
 The long term goals of our lab are to: (i) identify the repertoire of long noncoding RNAs that
participate p53-dependent physiological processes and (ii) characterize the molecular mechanisms of
these molecules with the goal of (iii) understanding how lncRNAs affect human health and disease in
order to modulate them for therapeutic gain. The research program outlined here represents the first
steps aimed at achieving these goals.

## Key facts

- **NIH application ID:** 10219299
- **Project number:** 5R35GM124909-05
- **Recipient organization:** SLOAN-KETTERING INST CAN RESEARCH
- **Principal Investigator:** Adam Schmitt
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $449,000
- **Award type:** 5
- **Project period:** 2017-08-01 → 2022-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10219299

## Citation

> US National Institutes of Health, RePORTER application 10219299, Investigating long noncoding RNAs as regulators of the p53 pathway functions. (5R35GM124909-05). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10219299. Licensed CC0.

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