# Defining the antibiotic intrinsic resistome of Acinetobacter baumannii

> **NIH NIH R01** · UNIVERSITY OF WASHINGTON · 2021 · $272,125

## Abstract

The project aims to define the functional basis of Acinetobacter baumannii intrinsic
resistance to three clinically-relevant antibiotics (meropenem, colistin, and tobramycin) at
a comprehensive scale. The work will identify resistance functions at genome-scale
using transposon mutant sequencing, and will validate results through single mutant
testing. The study will employ new technology that makes it possible to include mutants
inactivating essential genes, which is usually missing from such studies. Top, validated
mutants exhibiting increased sensitivity to one or more of the antibiotics will be further
evaluated for whether they act through established or novel resistance mechanisms.

## Key facts

- **NIH application ID:** 10220708
- **Project number:** 5R01AI148208-03
- **Recipient organization:** UNIVERSITY OF WASHINGTON
- **Principal Investigator:** Colin Manoil
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $272,125
- **Award type:** 5
- **Project period:** 2019-12-01 → 2023-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10220708

## Citation

> US National Institutes of Health, RePORTER application 10220708, Defining the antibiotic intrinsic resistome of Acinetobacter baumannii (5R01AI148208-03). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10220708. Licensed CC0.

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