# Biological Reagents

> **NIH NIH P50** · UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH · 2021 · $358,577

## Abstract

ABSTRACT
This Core provides peptide, protein, antibody and RNA reagents that support HIV/Host structural and
mechanistic studies within the CHEETAH Center. Bacterial expression systems will be the workhorse for
producing recombinant proteins without complex folding/post-translational modification requirements, while
eukaryotic expression systems will be employed to produce more complex proteins. Uniformly glycosylated or
deglycosylated proteins will be made using advanced tissue culture and enzymatic approaches.1-3 Membrane
proteins will be produced by coupling eukaryotic expression with comprehensive detergent screening
protocols.4 Peptide synthesis will be employed for the production of ligands, inhibitors, and protein fragments.
Larger synthetic peptides/proteins will be produced using native chemical ligation to enable the introduction of
site-specific labels and modifications.5 Finally, an automated high-throughput phage display screening and
characterization platform will be used to produce Fab antibody fragments for use as crystallography
chaperones, cryo-EM fiducials, biochemistry/cell biology tools, and to stabilize/trap heterogeneous protein
complexes in specific conformations for structural studies.6

## Key facts

- **NIH application ID:** 10221474
- **Project number:** 5P50AI150464-15
- **Recipient organization:** UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH
- **Principal Investigator:** Michael S Kay
- **Activity code:** P50 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $358,577
- **Award type:** 5
- **Project period:** 2007-08-27 → 2022-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10221474

## Citation

> US National Institutes of Health, RePORTER application 10221474, Biological Reagents (5P50AI150464-15). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/10221474. Licensed CC0.

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