# Spatial and temporal control of Rho family GTPases

> **NIH NIH R35** · UNIVERSITY OF CHICAGO · 2021 · $400,090

## Abstract

Many essential cellular functions require large scale organization of the cell cortex.
These include cytokinesis, asymmetric cell division, cell migration, and formation of an
epithelium. Extensive organization of the cytoskeleton and cell-cell junctions are
required during development to drive changes in the shape of cells, embryos, and
tissues. Each of these processes requires establishment and maintenance of
metastable domains that serve important cellular functions. The mechanisms by which
these domains assemble remain incompletely understood, in part due to the difficulty
inherent in functionally dissecting dynamic protein assemblies with current tools.
Recently developed optogenetic tools permit light-mediated control of protein activity in
live cells at high spatial and temporal resolution. This novel approach provides a
powerful means to dissect the underlying molecular mechanism underlying such
processes. We will combine the ability to alter the activity of specific molecules with high
spatial and temporal resolution with conventional cell biology, imaging, genetic, and
biochemical approaches, in order to better understand how cells divide, polarize and
shape tissues.

## Key facts

- **NIH application ID:** 10225352
- **Project number:** 5R35GM127091-04
- **Recipient organization:** UNIVERSITY OF CHICAGO
- **Principal Investigator:** Michael A. Glotzer
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $400,090
- **Award type:** 5
- **Project period:** 2018-08-01 → 2023-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10225352

## Citation

> US National Institutes of Health, RePORTER application 10225352, Spatial and temporal control of Rho family GTPases (5R35GM127091-04). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10225352. Licensed CC0.

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