# Core B: Immune Assessment

> **NIH NIH U19** · UNIVERSITY OF CALIFORNIA LOS ANGELES · 2021 · $73,214

## Abstract

CORE B: Immune Assessment
SUMMARY/ABSTRACT
The Immune Assessment Core B is essential to the research goals of the Project PIs collaborating in this HIPC
Consortium. The proposed research aims to characterize the innate and adaptive immune response to CMV in
recipients of heart, lung and renal allografts. Core B will provide skilled technical and scientific expertise to
enable the PIs to achieve their research goals through the following specific Aims. Specific Aim 1. To provide
professional expertise in the design and implementation of assays to measure immune cell function.
The Core has validated standardized 14-color flow cytometry, multiplex immunoassays, and KIR typing assays
to support the research of Projects 1 and 2. The Core will perform the assays and interpret the data. The IAC
will ensure consistent and reliable intra- and inter-assay performance using standardized protocols, reagents
and data interpretation. The core will coordinate experiments among the projects with the goal of maximizing
testing uniformity and cost reduction. Specific Aim 2. To provide standardized sample processing,
maintain a centralized biorepository of tissue, serum, plasma, peripheral blood mononuclear cells and
urine, and enable efficient sample and data sharing. Processing and storage of graft biopsies, PBMCs,
serum/plasma will be performed under standardized operating procedures honed by supporting clinical trials to
be HIPAA compliant. The BTM database will be used to house the sample inventories at both UCLA and
UCSF and associated data and made accessible to all PIs and staff. The Idea Elan core laboratory
management software will be used to facilitate work flow and electronic data reporting to Data Management
and Analysis Core B. Specific Aim 3. To provide education of PIs and staff in sample management,
immune assessment technologies and data analysis/interpretation, and to disseminate the new
knowledge in the HIPC Consortium. The Core will educate the investigators about technologies, their
applications and limitations, and will promote communication and collaboration among projects and cores.

## Key facts

- **NIH application ID:** 10225359
- **Project number:** 5U19AI128913-05
- **Recipient organization:** UNIVERSITY OF CALIFORNIA LOS ANGELES
- **Principal Investigator:** Maura Rossetti
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $73,214
- **Award type:** 5
- **Project period:** 2017-08-01 → 2024-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10225359

## Citation

> US National Institutes of Health, RePORTER application 10225359, Core B: Immune Assessment (5U19AI128913-05). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10225359. Licensed CC0.

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