# Novel role of beta2-adrenergic receptor signaling in vBNST CRF-mediated stress-induced ethanol intake

> **NIH NIH F31** · PENNSYLVANIA STATE UNIV HERSHEY MED CTR · 2021 · $5,386

## Abstract

PROJECT SUMMARY
Alcohol use disorder (AUD) affects about 16 million people in the United States. Unfortunately, despite the few
currently-available treatments, the rate of relapse is extraordinarily high. Stress is a common trigger of relapse;
therefore it is a prime target for AUD treatment. This proposal will investigate the combined effects of stress
and alcohol use on neurocircuitry in the limbic system, the primary group of structures involved in emotional
processing. The first specific aim of this proposal is to test the hypothesis that stress and ethanol interact to
promote beta2-adrenergic receptor (b2-AR) signaling in the bed nucleus of the stria terminalis (BNST) and
increase voluntary ethanol intake. This will be tested using whole-cell patch-clamp electrophysiology,
chemogenetic manipulations of BNST neurons, and fluorescent in situ hybridization. The second aim is to test
the hypothesis that targeting glucocorticoid receptor (GR)-mediated signaling in the BNST will mitigate b2-AR
dependent behavioral and neurocircuit changes following stress and ethanol intake. This will be achieved
through chromatin immunoprecipitation (ChIP), electrophysiology, and pharmacological manipulations of the
GR. Keeping consistent with the mission of the National Institute on Alcohol Abuse and Alcoholism (NIAAA),
this research proposal will investigate alcohol’s effect on health and well-being by identifying neurocircuitry
differences between stress and alcohol-exposed mice and naïve mice. Ultimately the findings from these
studies will help when developing prevention and treatments for stress-related alcohol use disorder.

## Key facts

- **NIH application ID:** 10226256
- **Project number:** 5F31AA027943-03
- **Recipient organization:** PENNSYLVANIA STATE UNIV HERSHEY MED CTR
- **Principal Investigator:** Angela E Snyder
- **Activity code:** F31 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $5,386
- **Award type:** 5
- **Project period:** 2019-09-01 → 2021-09-06

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10226256

## Citation

> US National Institutes of Health, RePORTER application 10226256, Novel role of beta2-adrenergic receptor signaling in vBNST CRF-mediated stress-induced ethanol intake (5F31AA027943-03). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10226256. Licensed CC0.

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