# Simplified Glycan Profiling Workflows of Captured Immune Glycoproteins and Cells

> **NIH NIH U01** · MEDICAL UNIVERSITY OF SOUTH CAROLINA · 2021 · $254,212

## Abstract

Abstract
The technologies available to rapidly and efficiently manipulate the expression of hundreds of
glycan biosynthetic genes to study the functional role of complex glycans in growing cells have
eclipsed the analytical capabilities to evaluate each cell phenotype in a comparative or
quantitative manner. Current glycome profiling approaches require specialized plate-based
sample handling resources, extensive processing and purification prior to analysis, and are
expensive in regards to processing time and enzyme. These are barriers to both non-glycomic
and glycomic researchers adopting large scale glycan analysis workflows applied to biofluids and
cells. Our collaborative group has recently developed a streamlined antibody capture slide array
approach to directly profile N-glycans of captured serum glycoproteins like IgG, a method
requiring a few microliters of sample and simplified processing workflows that require no
purification or sugar modifications prior to analysis. N-glycans are released from captured
glycoproteins and directly analyzed by MALDI-TOF mass spectrometry. We propose to expand
and adapt our slide array-based immune capture workflows to isolate immune cells directly from
biofluids, and provide rapid analysis workflows of cultured cells. The goal in these assays is to
develop rapid isolation workflows with minimal processing and direct glycan analysis, as
described in three Specific Aims: SA1. Development of an on slide method for glycan analysis of
immunoglobulin subtypes: SA 2. Development of Glyco-Cell Typer as applied to immune cell
sub-types. SA 3. Analysis of cultured cells on slides for direct glycan measurements. Optimization
of slide chemistry and processing will be emphasized, as well as conditions for metabolic isotope
labeling and quantitative glycan analysis. The goal will be to provide validated workflows such
that any research or core laboratory with a MALDI mass spectrometer will be able to perform
routine glycan analysis on the most common types of samples.

## Key facts

- **NIH application ID:** 10227699
- **Project number:** 5U01CA242096-03
- **Recipient organization:** MEDICAL UNIVERSITY OF SOUTH CAROLINA
- **Principal Investigator:** Peggi M Angel
- **Activity code:** U01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $254,212
- **Award type:** 5
- **Project period:** 2019-08-01 → 2022-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10227699

## Citation

> US National Institutes of Health, RePORTER application 10227699, Simplified Glycan Profiling Workflows of Captured Immune Glycoproteins and Cells (5U01CA242096-03). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10227699. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*