# Research Center for Spatiotemporal Lung Imaging and Omics

> **NIH NIH U01** · BATTELLE PACIFIC NORTHWEST LABORATORIES · 2021 · $936,387

## Abstract

PROJECT ABSTRACT
Alveologenesis characterizes the later stages of normal human lung development and is the final step of lung
maturation. Alveologenesis is a dynamic, coordinated process that requires the accurate spatial and temporal
integration of signals to develop the intricate alveolar structure. While important progress has been made,
significant knowledge gaps remain in our understanding of the molecular mechanisms underlying
alveologenesis. For this reason, NHLBI established Research Centers (RCs) to create a molecular atlas of the
developing lung (LungMAP) focused from birth up to early childhood (~8 years). In the first phase of
LungMAP, our RC developed resources that enabled a much more detailed understanding of normal lung
development up to early childhood. In LungMAP Phase 2, we will apply our successful approaches and our
newest technologies to extend analysis of lung development into early adulthood as well as human disease;
focusing on bronchopulmonary dysplasia (BPD), the most common morbidity of preterm infants which is
characterized by delayed or deficient lung maturation.
Within the lung, the relationship between space, anatomy, and function is fundamental. Therefore, our
approach includes new unbiased 3D quantitative imaging approaches implemented with high spatial
resolution, as well as cell-specific omics: proteomics (including activity-based proteomics and
phosphoproteomics), lipidomics, and metabolomics. The integration of these complementary data collection
methods facilitates the establishment of a cell-specific spatial atlas with an incredible breadth of molecular
profiles across the developing lung in normal and disease states.
Specifically, we will accomplish our goal of an integrated molecular atlas of lung development through the
following aims: (1) Spatial imaging for a molecular atlas of the human lung in normal and diseased states, (2)
Cell-specific omics for a molecular atlas of the human lung in normal and diseased states, and (3) Managing
data to facilitate collaboration and data integration.
Overall, these aims will create unprecedented multi-scale browsable quantitative three-dimensional “Google
Maps” of proteins, lipids, and metabolites across the developing lung, providing for many novel insights
toward understanding both normal human lung biology and disease pathogenesis.
The novel imaging approaches and the suite of integrated pan-omics capabilities (i.e. proteomics,
phosphoproteomics, activity-based proteomics, lipidomics and metabolomics) developed and available in a
single laboratory at PNNL represents a unique strength of the Research Center.

## Key facts

- **NIH application ID:** 10227737
- **Project number:** 5U01HL148860-03
- **Recipient organization:** BATTELLE PACIFIC NORTHWEST LABORATORIES
- **Principal Investigator:** Joshua N. Adkins
- **Activity code:** U01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $936,387
- **Award type:** 5
- **Project period:** 2019-09-01 → 2024-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10227737

## Citation

> US National Institutes of Health, RePORTER application 10227737, Research Center for Spatiotemporal Lung Imaging and Omics (5U01HL148860-03). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10227737. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*