# Rapid diagnosis and quantification of HIV by direct capture, labelling and detection of individual virions

> **NIH NIH R61** · DUKE UNIVERSITY · 2021 · $444,310

## Abstract

Abstract
We propose to develop an advanced rapid point of care diagnostic for HIV. This approach will
leverage a novel D4 diagnostic assay platform: The D4 is so named because of the chain of
events that occur upon addition of blood: (1) Dispense blood onto chip; (2) Dissolution of “soluble”
detection reagent spots (3) Diffusion of coated virion across a surface and binding to specific
capture Ab spots; (4) Detection of binding with fluorescence imaging. We will enhance HIV virion
capture using a lectin, Griffithsin, which can bind to the glycan shield of HIV virions spikes and
induce exposure of CD4 binding sites which can be captured by antibodies. Individual virions can
be captured, labelled and imaged using next generation cell phone based microscopy. This
diagnostic test enables not only rapid “window independent” diagnosis independent of patient
antibodies, but also potentially for routine viral load monitoring.

## Key facts

- **NIH application ID:** 10227771
- **Project number:** 5R61AI140485-03
- **Recipient organization:** DUKE UNIVERSITY
- **Principal Investigator:** Ashutosh Chilkoti
- **Activity code:** R61 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $444,310
- **Award type:** 5
- **Project period:** 2019-08-01 → 2023-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10227771

## Citation

> US National Institutes of Health, RePORTER application 10227771, Rapid diagnosis and quantification of HIV by direct capture, labelling and detection of individual virions (5R61AI140485-03). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10227771. Licensed CC0.

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