# Flow Cytometry, Microscopy, and Imaging Core

> **NIH NIH P20** · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · 2021 · $327,394

## Abstract

Abstract 
The Flow Cytometry, Microscopy, and Imaging (FCMI) Core is a continuation from Phase-1 aimed at providing 
a wide range of biotechnology equipment and technical expertise to the COBRE junior faculty pursuing research 
on Dietary Supplements and Inflammation. The Core will provide state-of-the-art equipment to the Target and 
Pilot Project Faculty and their lab personnel for performing cutting-edge research in an accurate and timely 
manner that would lead to successful completion of their proposed projects. One of the focus of this Core is to 
provide multi-parameter flow cytometry and sorting services for phenotyping and isolation of specific cell types, 
determination of status on cell proliferation, differentiation, apoptosis as well as expression of signaling 
molecules and cytokines at the protein level in inflammation and following treatment with botanicals. The Core 
will also offer a range of imaging systems for live and fixed cells, tissues and organisms, including labeled and 
unlabeled samples. In addition, image visualization, data acquisition, processing and analysis that would meet 
the needs of the COBRE investigators are provided. Examination of structural changes that occur in response 
to inflammation, and how various dietary supplements induce alterations is critical to the understanding of the 
mechanisms of immune dysfunction, and development of therapeutic and preventive strategies. Specifically, 
the Core will provide imaging facilities for monitoring these changes at the whole animal to molecular level. This 
shared resource will lead to development of biomarkers for early detection and intermediate end points in the 
progression of the disease as well as response to therapeutic modalities. Furthermore, the instruments and 
expertise will provide specific and sensitive assay systems for determination of various molecular signaling 
pathways. At the whole animal level, ultrasound and fluorescence techniques are available to determine how 
inflammation and dietary supplements affect the experimental animals. The optical microscopy includes confocal 
and multiphoton microscopy for high resolution imaging of tissues to subcellular processes within tissues of 
interest. Finally, ultrastructural changes in cells will be imaged by electron microscopy to determine changes at 
the sub-micron level of resolution. A full range of image analysis hardware and software is also available to 
quantify the structural changes which occur during inflammation in the cells, tissues, and organs and how these 
changes are affected by the various dietary supplements. All equipment and technical expertise is available at 
USC SOM through outstanding institutional support. The Core faculty and staff will be available for assistance 
with experimental design, operation of equipment, and training on imaging instrumentation. In addition to 
individual interactions, didactic courses, seminars and workshops are regularly sponsored by the Instr...

## Key facts

- **NIH application ID:** 10227908
- **Project number:** 5P20GM103641-09
- **Recipient organization:** UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
- **Principal Investigator:** Narendra P Singh
- **Activity code:** P20 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $327,394
- **Award type:** 5
- **Project period:** 2012-09-01 → 2023-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10227908

## Citation

> US National Institutes of Health, RePORTER application 10227908, Flow Cytometry, Microscopy, and Imaging Core (5P20GM103641-09). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10227908. Licensed CC0.

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