# Neuronal ion and volume shifts after acute brain injury

> **NIH NIH R35** · MASSACHUSETTS GENERAL HOSPITAL · 2021 · $124,328

## Abstract

PROPOSAL ABSTRACT
We propose to test the accuracy and feasibility of a new, time-resolved measure of cell death after
organophosphate (OP) poisoning in a murine model of OP-induced status epilepticus. This novel
assay was developed as part of the grant, “Mechanisms of cell death during epileptogenesis”, a 7-
year R37 (Javits Award) project. We hypothesize that single-timepoint assays of cell death after OP
poisoning are not accurate because they cannot determine 1) how many cells have already
undergone necrosis and are no longer visible, 2) how many cells have irreversibly committed to
apoptotic death but are not yet positive for biomarkers of that pathway, and 3) how many cells have
already undergone apoptotic death and have been phagocytosed by microglia so that they are no
longer visible. We propose to measure cell death by the transgenic fluorescent protein
quenching assay before and after OP poisoning to determine both the extent and the timing of
neuronal death. These studies will form critical preliminary data for subsequent rigorous studies
measuring the causative role of seizures in neuronal death and the neuroprotective value of various
anticonvulsant and other treatment strategies after OP poisoning.

## Key facts

- **NIH application ID:** 10228299
- **Project number:** 3R35NS116852-01S1
- **Recipient organization:** MASSACHUSETTS GENERAL HOSPITAL
- **Principal Investigator:** Kevin J. Staley
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $124,328
- **Award type:** 3
- **Project period:** 2020-12-01 → 2021-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10228299

## Citation

> US National Institutes of Health, RePORTER application 10228299, Neuronal ion and volume shifts after acute brain injury (3R35NS116852-01S1). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10228299. Licensed CC0.

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