# Proteomic Approaches to HIV Function

> **NIH NIH P50** · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · 2021 · $317,180

## Abstract

The goal of Core 1 is to provide mass spectrometry-based proteomic platforms for global and targeted
characterization of HIV and host proteins in support of the aims and efforts for Projects 1-7 of the HARC
Center. We are continuously developing new innovative strategies and improvements for more sensitive and
quantitative analysis of protein abundance and posttranslational modifications, as well as for protein-protein
interactions (PPIs) and protein structure determination. Our affinity-purification mass spectrometry (AP-MS)
capabilities, along with newly developed proximity-based biotinylation (APEX-MS) and cross-linking mass
spectrometry (XL-MS) platforms, will aid research in Projects 1-6 through interaction network mapping for Vif,
Vpu, Nef, Tat, and Rev proteins in the context of HIV infection, as well as new factors identified through our
unique evolutionary analysis in Project 7. Also, our new global PTM analyses pipeline will provide direct
support for researchers in Projects 2, 3 and 5, focused on Vif, Vpu and Tat, respectively. Core 1 will work
closely with Core 5, which will focus on computationally analyzing all the proteomic data generated. In
addition, our XL-MS capabilities will also collaborate with Core 5 for structural determination and protein-interface
mapping analysis for Projects 1, 3, 4 and 5. The expertise, highly sensitive MS-analysis, and ongoing
innovations provided by Core 1 will continue to serve and adapt to the needs of Projects and Cores of the
HARC Center.

## Key facts

- **NIH application ID:** 10229560
- **Project number:** 5P50AI150476-15
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
- **Principal Investigator:** Nevan J Krogan
- **Activity code:** P50 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $317,180
- **Award type:** 5
- **Project period:** 2007-08-27 → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10229560

## Citation

> US National Institutes of Health, RePORTER application 10229560, Proteomic Approaches to HIV Function (5P50AI150476-15). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/10229560. Licensed CC0.

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