# Molecular Imaging Using Cryo-EM

> **NIH NIH P50** · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · 2021 · $297,506

## Abstract

As a versatile structure determination technology, cryo-EM is an integrated part of the HARC Center. In the
past funding cycle, the Cryo-EM Core has engaged in technology development of single-particle cryo-EM, and
has made major contributions to the recent technological breakthroughs in single particle cryo-EM that has led
to a resolution revolution. Briefly, we developed one of the very first programs to correct beam-induced image
motion, developed a novel approach of using conformation-specific Fabs to facilitate single particle cryo-EM
studies, determined the first atomic structure of an integral membrane protein that changed the perspective of
the entire structural biology community, determined atomic structures of integral membrane proteins in lipid
nanodiscs and developed a novel saposin-based lipid nanoparticle system for single particle cryo-EM studies
of small integral membrane proteins. In this renewal, we will both focus on methodology development as well
as application of state-of-the-art single particle cryo-EM technology to solve critical HIV structural biology
questions.

## Key facts

- **NIH application ID:** 10229563
- **Project number:** 5P50AI150476-15
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
- **Principal Investigator:** Yifan Cheng
- **Activity code:** P50 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $297,506
- **Award type:** 5
- **Project period:** 2007-08-27 → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10229563

## Citation

> US National Institutes of Health, RePORTER application 10229563, Molecular Imaging Using Cryo-EM (5P50AI150476-15). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10229563. Licensed CC0.

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