# Fluorescence Microscopy and Cell Imaging Shared Resource

> **NIH NIH P30** · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · 2020 · $6,765

## Abstract

FLUORESCENCE MICROSCOPY & CELL IMAGING SHARED RESOURCE 
ABSTRACT 
The Fluorescence Microscopy and Cell Imaging Resource offers complete fluorescence microscopy imaging 
services. This Cancer Center managed resource is dedicated to enabling innovative, significant cancer research 
and promoting trainee development. The Resource provides member access to cost-effective services, 
sophisticated instrumentation, and salient expertise in quantitative, biomolecular imaging of cells and tissues. 
Faculty Director Angela Wandinger-Ness, PhD supervises the Resource, which is operated by expert staff with 
38 years combined experience in light microscopy. Resource instrumentation is centrally located on the second 
floor of the Cancer Research Facility and a Cancer Center managed, web-based reservation system (iLabs) 
tracks usage and billing. Cutting-edge technologies for biomolecular imaging at super-resolution (nm scale), 
hyperspectral imaging for four-dimensional particle tracking, and light sheet microscopy (in development) are 
offered through negotiated agreements. More routine applications are readily accommodated by an array of 
upright and inverted Zeiss, Nikon and Olympus microscopes. Two Zeiss META confocal scanheads as well as 
an Olympus DSU spinning disk system enable confocal and multi-photon imaging of both fixed and live cells and 
tissues. Laser and scanhead configurations allow imaging of up to eight colors in the visible range, FRET, FRAP 
and infrared excitation. A CRi Nuance spectral camera, an Andor iXon EMCCD and a new Hamamatsu Flash 
4.0 sCMOS camera offer exceptional high speed and resolution, low light sensitivity, and spectral image 
collection on wide-field instruments. TIRF capabilities for visualization of membrane events, single particle 
tracking and imaging in custom microfluidics chambers round out the top-flight capabilities of the Shared 
Resource. Instrumentation is regularly upgraded, in accordance with member needs, and through successful 
solicitation of extramural funding and UNMCC support, e.g. multi-photon instrument newly acquired in previous 
funding cycle. Multiple software packages allow image deconvolution, stereology, and quantitative evaluation of 
all collected data. Infrequent users may request full service imaging by expert staff members. Fees are 
comparable to other institutions. Resource personnel promote excellence in cancer research by incorporating 
the latest innovative technologies and instrumentation and by giving personalized advisement on best practices 
for sample preparation, probe selection and quantitative measures. Education and dissemination about resource 
capabilities is multi-modal: ranging from web-based resources and tours to custom, targeted lectures, and hands- 
on graduate courses. During the previous 5-yr funding period, 48 Cancer Center laboratories from all 4 UNMCC 
Research Programs used the Resource, resulting in a total of 89 publications, of which 12 are presently pend...

## Key facts

- **NIH application ID:** 10230656
- **Project number:** 3P30CA118100-15S9
- **Recipient organization:** UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR
- **Principal Investigator:** CHERYL LYNN WILLMAN
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $6,765
- **Award type:** 3
- **Project period:** 2005-09-26 → 2021-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10230656

## Citation

> US National Institutes of Health, RePORTER application 10230656, Fluorescence Microscopy and Cell Imaging Shared Resource (3P30CA118100-15S9). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10230656. Licensed CC0.

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